FastQCFastQC Report
Fri 31 Oct 2014
AH8038D-141013_GTGAAA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8038D-141013_GTGAAA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12610238
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG248670.19719691254042945TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA70400.035.8006481
ACACGTC68950.035.5025213
GCACACG68800.035.31844711
ACGTCTG71050.034.92818515
CGTCTGA69650.034.9195916
AGTCACG62100.034.85556428
GTCACGT64800.034.54841629
CACGTCT71600.034.50276614
CACGTGA65050.033.86128231
CACACGT72000.033.77998712
TCGGAAG76350.033.1224063
TCACGTG68650.032.60972230
CTCGTAT63700.032.18246544
ATCGGAA79400.031.5667082
TCTCGTA66300.031.15799343
ATCTCGT66550.030.90532542
GATCTCG68650.030.38581841
AGCACAC79450.030.1876710
CGGAAGA83100.030.0797444
TCGTATG70800.029.81329245
Produced by FastQC (version 0.11.2)