FastQCFastQC Report
Fri 31 Oct 2014
AH8038C-141013_GCCAAT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8038C-141013_GCCAAT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23686796
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC28300.024.8840829
CTCGGGG17100.023.55166216
CCTCGGG18150.022.06525415
GCACACG37000.021.40456811
TCACGCC33200.021.00808130
GGCGGTC22650.019.96779638
GATCGGA41300.019.5623861
ACGTCTG43650.018.45288815
ACACGTC43050.018.29196713
AGTCACG39250.018.2861828
CACACGT43800.017.92738312
TCGGGGA26350.017.84556617
CACGCCA40000.017.60545231
CCAGGCG28550.016.62796835
CGTCTGA48900.016.42570516
CACGTCT48800.016.22887414
CCCAGGC28250.016.00812334
AGCACAC51550.015.71222110
CAGGCGG30800.015.55935936
TATCTCG48800.015.30871839
Produced by FastQC (version 0.11.2)