FastQCFastQC Report
Fri 31 Oct 2014
AH8038B-141013_ATGTCA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8038B-141013_ATGTCA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16116054
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1506200.9345960245603545TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG196050.041.45191211
GATCGGA203800.040.4963531
ACACGTC202050.040.22084813
CACACGT203450.039.944212
CACGTCT205450.039.5880814
ACGTCTG205400.039.58676515
CGTCTGA206350.039.43722516
AGCACAC209000.039.04483410
GAACTCC208850.038.6541821
CAGTCAC209400.038.33787527
GAGCACA214950.038.141989
CTCGTAT203550.038.0420144
TCACATG209600.037.9883330
AGAGCAC216950.037.8629538
CACATGT210600.037.80795331
ATCTCGT207450.037.76074242
TCCAGTC213100.037.58775325
GTCACAT212550.037.5670629
TCGGAAG220700.037.5055163
TCGTATG205750.037.36239245
Produced by FastQC (version 0.11.2)