FastQCFastQC Report
Fri 31 Oct 2014
AH8038B-141013_ATGTCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8038B-141013_ATGTCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10188809
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG526690.5169298982834991TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA123050.040.140331
GCACACG119350.040.04019511
ACACGTC122600.039.2907513
CACACGT122200.039.05112512
ACGTCTG125950.038.8171415
CGTCTGA122250.038.6484816
CACGTCT126200.038.49083714
TCGGAAG130900.037.6752783
AGCACAC125300.037.56424710
GAGCACA126500.037.4739579
CAGTCAC119850.037.0027527
ATCGGAA131950.037.000362
AGAGCAC128100.036.935658
CCAGTCA118950.036.8476626
GAACTCC121250.036.74233221
CTCGTAT113950.036.55580544
CACATGT121600.036.45011531
GTCTGAA127200.036.3654917
TCTCGTA115200.036.33496543
TCCAGTC125150.036.3166625
Produced by FastQC (version 0.11.2)