FastQCFastQC Report
Fri 31 Oct 2014
AH8038A-141013_AGTTCC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8038A-141013_AGTTCC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17655380
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2608701.4775666114238266TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG305450.042.83977511
GATCGGA313200.042.1935961
ACACGTC310950.042.10362213
ACGTCTG312200.041.8629815
CACACGT313050.041.7997412
CACGTCT315600.041.3834714
CGTCTGA316000.041.26700616
AGCACAC319350.041.1087610
AGTTCCG310450.040.82322334
CAGTCAC317350.040.7381327
GAGCACA322200.040.7172059
TCCAGTC319300.040.5034325
GAACTCC321550.040.42981321
CCGTATC310350.040.40529638
TATCTCG311850.040.3599441
TCGGAAG327850.040.297213
GTTCCGT314600.040.1345335
CCAGTCA322350.040.09227826
AGTCACA322300.040.077128
GTCACAG321850.040.0065129
Produced by FastQC (version 0.11.2)