FastQCFastQC Report
Fri 31 Oct 2014
AH8038A-141013_AGTTCC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8038A-141013_AGTTCC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11595492
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG909430.7842961730300018TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA189000.042.1471061
GCACACG186400.041.8353511
ACACGTC188200.041.3754513
ACGTCTG190650.041.3274115
CACACGT188400.041.2837612
CACGTCT190750.041.07002614
CGTCTGA187500.041.04976716
AGTTCCG172850.040.06400734
GAACTCC180900.040.0615621
AGCACAC190300.039.96121210
CCGTATC165700.039.93880538
GAGCACA191400.039.93079
TCGGAAG198200.039.877313
TATCTCG173550.039.7963841
CAGTCAC175650.039.74814627
TCCAGTC182250.039.72846225
AGAGCAC193100.039.5675058
GTTCCGT181950.039.5317235
ATCGGAA199200.039.4399452
CCAGTCA176450.039.26189826
Produced by FastQC (version 0.11.2)