FastQCFastQC Report
Fri 31 Oct 2014
AH7814B-141013_GCCAAT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7814B-141013_GCCAAT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6958298
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC647640.9307448459379003TruSeq Adapter, Index 6 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA272740.3919636669771832No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC219100.31487585038755167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG76800.041.7756511
GTCACGC77050.041.08499529
GATCGGA79800.040.9796371
CACACGT79450.040.46720512
ACGTCTG79450.040.43888515
AGTCACG78350.040.43260228
TCACGCC77850.040.43101530
CACGTCT79950.040.38297714
ACACGTC80350.040.0979413
CTCGGGG26050.039.9024116
CGTCTGA81200.039.56736416
CACGCCA79700.039.464331
AGCACAC82350.039.15029510
CCTCGGG26600.039.07736215
TATCTCG80700.039.03692239
GAGCACA84050.038.4919979
GTATGCC83250.038.3354445
GAACTCC83450.038.12335221
TCGGGGA33700.037.92103617
GGCGGTC33600.037.90324438
Produced by FastQC (version 0.11.2)