FastQCFastQC Report
Fri 31 Oct 2014
AH7814A-141013_ACAGTG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7814A-141013_ACAGTG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5162396
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC70777713.710242298343637TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA761850.044.6992151
GCACACG753550.044.62651411
CACACGT755700.044.4914812
ACACGTC755450.044.45557413
TCGGAAG764900.044.43913
ACGTCTG754750.044.40439615
AGCACAC759000.044.3945710
AGAGCAC761400.044.3863148
CACGTCT755100.044.37785314
GAGCACA761000.044.3712129
ATCGGAA767850.044.3328252
CGTCTGA755450.044.30666416
GTATGCC751600.044.13377845
CGGAAGA770000.044.1301544
GTCTGAA760900.044.0146517
GAACTCC757400.044.01140221
GAAGAGC771050.043.9591836
TCTGAAC762000.043.906418
TCCAGTC754750.043.9048725
ACTCCAG757000.043.8873223
Produced by FastQC (version 0.11.2)