FastQCFastQC Report
Fri 31 Oct 2014
AH7688B-141013_TGACCA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7688B-141013_TGACCA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110549
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC594960.836728640784277TruSeq Adapter, Index 4 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA97470.13707802308935638No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC88730.12478642647705543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG72550.040.40759311
ACACGTC73150.039.8921813
GATCGGA74250.039.739661
CACACGT73750.039.5676312
CACGTCT73600.039.55656414
ACGTCTG74100.039.16819815
GAGCACA76550.038.383259
CGTCTGA75600.038.3612916
TCCAGTC75550.038.21095325
AGCACAC76800.038.17122710
ACTGACC75250.038.12086532
GAACTCC76100.038.0527921
GTATGCC75400.037.5771145
CAGTCAC76950.037.48651527
AGAGCAC78650.037.4728168
CCAGTCA77300.037.28767826
ATCTCGT77200.036.99116540
TCGGAAG80150.036.8559843
AGTCACT78450.036.85502228
CTCGTAT78150.036.37000742
Produced by FastQC (version 0.11.2)