FastQCFastQC Report
Fri 31 Oct 2014
AH7688A-141013_CGATGT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7688A-141013_CGATGT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5281760
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC157310.29783632728484444TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21600.035.93575311
GATCGGA22550.034.628261
ACACGTC22350.034.5291813
CACACGT22550.034.42248512
CACGTCT23400.032.9797914
ACGTCTG24350.031.50830715
AGCACAC24750.031.45242110
CAGTCAC23850.031.41505427
CGTCTGA24650.031.39866416
TATCTCG23900.031.35319339
CCGATGT23250.031.06271233
GAGCACA25850.030.4615829
AGAGCAC26250.029.9974088
GTATGCC25250.029.23460645
TCCAGTC26350.029.20299725
GTCACCG25750.029.09594729
AGTCACC25700.028.8907428
GAACTCC26350.028.86144321
CACCGAT26000.028.38323631
ATCTCGT26200.028.34475740
Produced by FastQC (version 0.11.2)