FastQCFastQC Report
Fri 31 Oct 2014
AH7669B-141013_CCGTCC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7669B-141013_CCGTCC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015696
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG421361.0492826150186667TruSeq Adapter, Index 16 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA207560.5168717950761212No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC157060.3911152637052207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG49100.042.56907735
GCACACG51950.042.3114811
ACCCGTC50650.041.84383832
GATCGGA53400.041.812251
GTCCCGA49150.041.74758536
CCCGTCC50800.041.45454833
TCACCCG51350.041.44919630
CACGTCT53200.041.36012614
CACCCGT51550.041.24422531
CACACGT53350.041.20166412
ACGTCTG53400.041.16308615
ACACGTC53950.040.8268513
AGCACAC54100.040.79631810
CCGTCCC51750.040.5196434
CCCGATC50150.040.5179238
CGTCTGA54300.040.48082716
AGAGCAC54900.040.3652578
TCCCGAT50800.040.2174637
GAGCACA55000.040.169159
GTCACCC53100.040.12604529
Produced by FastQC (version 0.11.2)