FastQCFastQC Report
Fri 31 Oct 2014
AH7669A-141013_ATGTCA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7669A-141013_ATGTCA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5144637
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG505220.9820323571906046TruSeq Adapter, Index 15 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA448210.8712179304390184No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC352790.685743231252273No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC75710.1471629582417574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG62700.042.23276511
CTCGGGG37500.042.11675316
GATCGGA64200.041.7854771
GGCGGTC45350.041.47984738
CCTCGGG38050.041.4488415
CTGACCC52400.041.4437540
TAAGCCG52800.041.28943316
AGCACAC64350.041.25395610
ACGTCTG64200.041.10664415
CACGTCT64200.041.10664414
CACACGT64550.041.09248412
ACACGTC64500.041.0196913
CGTCTGA64450.040.9471916
TCGGGGA46300.040.8661817
CCTGACC53350.040.74279839
TCCAGGG53700.040.637354
CCAGGGA53900.040.52835
CAGTCAC65300.040.24817727
CCCAGGC47050.040.1202934
AGCCGAT54400.040.07425318
Produced by FastQC (version 0.11.2)