FastQCFastQC Report
Fri 31 Oct 2014
AH7661C-141013_TGACCA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7661C-141013_TGACCA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4468315
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC506761.1341187897451275TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG60200.041.26111
GATCGGA61450.041.0574381
CACACGT60500.040.90764612
ACGTCTG60400.040.9008715
ACACGTC60850.040.74630413
CGTCTGA60800.040.59478416
CACGTCT61600.040.25020214
AGCACAC63250.039.44875710
GAGCACA63650.039.377599
ACTGACC60750.039.29446832
GAACTCC62400.039.23069821
GTATGCC59600.039.15411845
CAGTCAC61750.039.13353727
TCCAGTC62300.038.8241725
TCGGAAG65250.038.7917373
CCAGTCA62350.038.7569526
GTCTGAA64050.038.57006517
CTCGTAT61150.038.4928642
AGAGCAC65200.038.4764028
CGTATGC60800.038.45536444
Produced by FastQC (version 0.11.2)