FastQCFastQC Report
Fri 31 Oct 2014
AH7661C-141013_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7661C-141013_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724900
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC151990.5577819369518148TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG36150.041.38899611
GATCGGA38700.041.1662861
CACGTCT37300.041.01772714
ACGTCTG37650.040.99497615
ACACGTC36950.040.7364513
CACACGT36900.040.5477612
CGTCTGA37650.040.27786616
TCCAGTC35050.039.99332425
GAGCACA37800.039.758719
TCGGAAG40150.039.392853
CCAGTCA34650.039.35109726
AGCACAC37750.039.2764310
AGAGCAC38850.039.0316128
CAGTCAC35650.038.68908327
GTCTGAA39400.038.60166517
GAACTCC37000.038.4936821
GTATGCC32700.038.12949845
AGTCACT36900.038.1094928
ACTGACC35850.037.90490332
CTCGTAT34750.037.62811342
Produced by FastQC (version 0.11.2)