FastQCFastQC Report
Fri 31 Oct 2014
AH7661B-141013_ACAGTG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7661B-141013_ACAGTG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9527798
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC5502565.775269374938469TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG605350.044.16596211
GATCGGA614400.044.0650181
ACACGTC607300.043.9607113
CACACGT608200.043.94790612
CACGTCT608100.043.8288814
ACGTCTG608750.043.78577415
AGCACAC612550.043.76781510
GAGCACA614450.043.6983879
CGTCTGA609550.043.6655616
TCGGAAG620300.043.5725823
AGAGCAC617400.043.529688
GTATGCC604850.043.40461745
ATCGGAA624200.043.3915942
GTCACAC607400.043.38303429
GTCTGAA614400.043.36847317
GAACTCC612500.043.27639421
GATCTCG607500.043.12600339
CGGAAGA626200.043.111524
CAGTCAC612200.043.0989227
CCAGTCA611300.043.0742626
Produced by FastQC (version 0.11.2)