FastQCFastQC Report
Fri 31 Oct 2014
AH7661B-141013_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7661B-141013_ACAGTG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6242214
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1827622.9278393851924975TruSeq Adapter, Index 5 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGCTCTCGTATGCC107010.1714295600887762TruSeq Adapter, Index 5 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTCTGCC103750.16620705409971526TruSeq Adapter, Index 5 (98% over 51bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACACAGTGATCTCGTATGCC91040.14584568872518627TruSeq Adapter, Index 5 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA380600.044.1852871
GCACACG365850.044.0271711
ACACGTC366950.043.9565113
CACACGT366650.043.78383312
ACGTCTG372350.043.7715215
CACGTCT372750.043.6286714
AGCACAC361200.043.5973110
GAGCACA365450.043.5752759
TCGGAAG380650.043.5722583
CGTCTGA363000.043.39902516
AGAGCAC367200.043.3737348
ATCGGAA381800.043.3231622
GAACTCC343050.043.18314721
GTCTGAA360400.043.1488817
GTATGCC328200.043.0348445
GTCACAC345550.043.02630629
TCTGAAC358300.042.91199518
GATCTCG342950.042.858739
TCCAGTC350450.042.83629225
AGTCACA337900.042.80254428
Produced by FastQC (version 0.11.2)