FastQCFastQC Report
Fri 31 Oct 2014
AH7661A-141013_CGATGT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7661A-141013_CGATGT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6556832
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC452300.6898148374092855TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG56600.040.5464611
ACACGTC57250.040.00750713
GATCGGA58450.039.6938131
CACACGT58300.039.2869612
ACGTCTG58850.038.99625415
CGTCTGA60000.038.3610316
TATCTCG58750.038.26365339
CACGTCT60250.038.0154314
AGCACAC60600.037.9440910
CCGATGT60050.037.54199233
TCCAGTC60650.037.3580725
GAGCACA61750.037.346759
GTATGCC59700.037.2033145
CACCGAT61250.037.10033831
CGATGTA61800.036.77015734
CAGTCAC61700.036.72231727
AGAGCAC63200.036.596428
GAACTCC61950.036.3925221
GTCACCG62500.036.3586129
CGTATGC60850.036.35200544
Produced by FastQC (version 0.11.2)