FastQCFastQC Report
Fri 31 Oct 2014
AH7661A-141013_CGATGT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7661A-141013_CGATGT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4378780
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC148850.3399348677028762TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG33550.038.96112415
GATCGGA34200.038.8206751
GCACACG33500.038.68348311
ACACGTC33900.037.9615813
CACACGT34400.037.8022212
CGTCTGA34550.036.92180616
CACGTCT35600.036.65438514
CAGTCAC33050.035.5370727
ACCGATG32400.035.34480732
TCCAGTC34250.035.14599225
GAGCACA36500.035.1341869
AGAGCAC36850.035.04478
TATCTCG32300.035.0443139
AGCACAC36800.034.7866310
GTCTGAA36350.034.7221317
CACCGAT32450.034.66635531
CCGATGT33100.034.66530633
GAACTCC34650.034.61039721
CCAGTCA34000.034.61030226
CGATGTA32000.034.59139334
Produced by FastQC (version 0.11.2)