FastQCFastQC Report
Fri 31 Oct 2014
AH7011C-141013_AGTCAA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7011C-141013_AGTCAA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4058649
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG79120.1949417158271139TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12450.031.6253211
GATCGGA13000.030.9872421
ACGTCTG13700.028.9036715
AGCACAC13850.028.75343310
CACACGT13750.028.63528812
CACGTCT14350.028.06480614
CGTCTGA14750.027.4562616
ACACGTC14600.027.43014113
CAGTCAC14250.027.31611327
AGTCACA14700.025.86765328
GAGCACA15850.025.5510949
GTCACAG15450.024.75635529
CCAGTCA16000.024.75029226
GAACTCC16050.024.67318721
CTCGTAT15250.024.6426744
AGAGCAC16500.024.5448368
TCGGAAG16900.023.9635983
TCCAGTC16850.023.63529425
CACAGTC15900.023.63089831
TCTCGTA16700.023.04203243
Produced by FastQC (version 0.11.2)