FastQCFastQC Report
Fri 31 Oct 2014
AH7011C-141013_AGTCAA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7011C-141013_AGTCAA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510873
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG25900.10315137404400779TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG8450.029.0224611
ACGTCTG8300.029.0048215
GATCGGA8650.028.8750821
CACGTCT8500.028.3223514
CACACGT8600.027.99302112
ACACGTC8850.026.94803213
CGTCTGA9000.025.99891916
GAACTCC9000.025.5009721
TCGGAAG9850.025.3542773
AGCACAC9500.025.34105110
CTCGTAT8900.024.52535844
GAGCACA9950.024.4210959
AGAGCAC10000.024.298998
CAGTCAC9300.024.1944727
TCCAGTC9550.024.0323325
GTCACAG9850.022.61462429
ACTCCAG11100.022.50085623
CACAGTC9050.022.37475831
ATCGGAA11050.022.1936472
GGCGGTC3000.021.75082838
Produced by FastQC (version 0.11.2)