FastQCFastQC Report
Fri 31 Oct 2014
AH7011B-141013_CTTGTA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7011B-141013_CTTGTA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6131689
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC333950.544629709693365TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG42450.039.8040711
GATCGGA44150.038.2798351
CACACGT44700.037.59917412
ACACGTC44800.037.51524413
ACGTCTG45250.037.14216215
CACGTCT46300.036.49422514
AGCACAC46550.036.34656510
CGTCTGA46550.036.15323316
GTATGCC46300.035.28656445
GAGCACA48000.035.201729
CAGTCAC47400.035.0785727
CGTATGC46800.035.0051744
GTCACCT47700.034.6212629
GAACTCC48700.034.51150521
TCGGAAG49450.034.443633
AGTCACC48300.034.37779228
ATCTCGT48150.034.11635640
CTCGTAT47900.034.06035242
TCCAGTC49450.033.85185225
CCAGTCA49250.033.76090226
Produced by FastQC (version 0.11.2)