FastQCFastQC Report
Fri 31 Oct 2014
AH7011B-141013_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7011B-141013_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3960528
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC116750.2947839277995257TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA26200.038.4800341
GCACACG27100.037.44270711
ACACGTC27550.036.58612413
ACGTCTG28250.036.2370615
CACACGT28050.036.1745912
CACGTCT28600.035.55759414
CGTCTGA28400.034.93656516
GAACTCC26800.034.33850521
GTATGCC25250.033.9560245
CTCGTAT25750.033.90757442
GAGCACA29900.033.7101979
ATCTCGT26150.033.6462240
AGCACAC29700.033.48311210
TCGGAAG30800.033.3825873
AGTCACC27300.033.1326628
TCCAGTC28300.033.07541325
CAGTCAC27200.033.00671827
TCTCGTA26750.032.55546641
ATCGGAA31150.032.5019152
CGTATGC26750.032.30432544
Produced by FastQC (version 0.11.2)