FastQCFastQC Report
Fri 31 Oct 2014
AH7011A-141013_CAGATC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7011A-141013_CAGATC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6857913
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC228340.33295843793877233TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG31700.036.48185711
GATCGGA33900.034.852511
ACGTCTG33800.034.4817515
CACACGT33650.034.36800412
CACGTCT34000.034.14656414
ACACGTC34050.034.03034613
CGTCTGA34700.033.5225716
CATCTCG33500.032.84520339
CAGTCAC34750.032.17915727
AGCACAC36300.031.98231110
GAGCACA37350.031.2036869
AGAGCAC37650.031.1343298
GAACTCC36750.031.10136821
TCCAGTC36600.030.7370425
CCAGTCA36600.030.6140926
AGTCACC36750.030.61158228
CGTATGC37250.029.96301544
GTATGCC37300.029.86295745
CTCGTAT37200.029.51936142
ATCTCGT37850.029.01200140
Produced by FastQC (version 0.11.2)