FastQCFastQC Report
Fri 31 Oct 2014
AH7011A-141013_CAGATC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH7011A-141013_CAGATC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4465141
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC71840.16089077590158968TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20300.034.357911
ACACGTC20300.034.13623413
GATCGGA21200.034.0701331
CACACGT21150.032.9770812
ACGTCTG21050.032.91997515
CACGTCT21600.032.185914
AGCACAC21600.031.24844710
CGTCTGA22300.030.87290416
TCGGAAG23750.030.5981353
GAGCACA22650.030.594169
CATCTCG19550.030.15701939
AGAGCAC23600.029.2672828
TCCAGTC21250.029.22371125
GAACTCC22300.028.45309321
CAGTCAC20850.028.38147227
GTCTGAA24100.028.19330217
GTATGCC20150.027.58575845
CCAGTCA21750.027.51741226
AGTCACC21000.027.32159628
CGTATGC20950.027.28429444
Produced by FastQC (version 0.11.2)