FastQCFastQC Report
Fri 31 Oct 2014
AH6704F-141013_GGTAGC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6704F-141013_GGTAGC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5513413
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC721151.3079919824616804TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG84500.042.49495311
GTCACGG83850.042.10027729
GATCGGA86350.042.0891231
AGTCACG84750.041.67974528
ACACGTC86950.041.3245713
CACACGT86950.041.2469412
ACGTCTG87500.041.03909715
AGCACAC88350.040.7701210
CACGTCT88550.040.5778814
TCACGGT86300.040.53972230
CATCTCG86600.040.5350739
CACGGTA86650.040.4538731
CGGTAGC86400.040.4146833
GAACTCC88100.040.1722221
CGTCTGA88900.040.06379716
AGAGCAC90400.039.8942688
CAGTCAC88650.039.87222327
GAGCACA90200.039.8580139
GTATGCC86800.039.77341545
CCAGTCA89250.039.6545926
Produced by FastQC (version 0.11.2)