FastQCFastQC Report
Fri 31 Oct 2014
AH6704E-141013_GAGTGG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6704E-141013_GAGTGG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6521482
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC516690.7922892373236635TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG63800.040.0946811
CACGAGT63150.039.90172631
GTCACGA64550.039.31574629
CGAGTGG63800.039.24805533
AGTCACG64900.039.0024128
ACGAGTG65000.038.90421332
ACGTCTG66400.038.59425415
GATCGGA67200.038.545731
CGTCTGA66900.038.33943616
CACACGT66850.038.26714312
TCACGAG66250.038.23867430
ACACGTC66950.038.14277313
GGATCTC65550.037.93148438
GATCTCG65950.037.73670239
CAGTCAC67450.037.66132727
CACGTCT67950.037.61454814
GTGGATC66100.037.40588436
GAGCACA68600.037.2880829
CGTATGC67850.036.95406344
GTATGCC67800.036.84852645
Produced by FastQC (version 0.11.2)