FastQCFastQC Report
Fri 31 Oct 2014
AH6704D-141013_CGTACG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6704D-141013_CGTACG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6811775
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC851451.2499678864906725TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG98650.041.2572311
GATCGGA100200.041.191551
CCGTACG95750.041.0020633
ACACGTC100450.040.42892513
CGTACGA96700.040.3433234
CACGTCT101200.040.12930714
AGCACAC101950.040.0756710
CACACGT101600.040.059912
GAGCACA102400.040.0085339
GATCTCG97700.039.93830539
ACGTCTG101750.039.86816415
CGTCTGA102200.039.6706116
CGATCTC98100.039.6125638
AGAGCAC104300.039.2365728
TCGGAAG105550.039.1351173
CAGTCAC102400.038.93540227
TCCAGTC103150.038.89224225
GTATGCC101400.038.75298345
CCAGTCA103350.038.72989726
ACCGTAC102100.038.71640432
Produced by FastQC (version 0.11.2)