FastQCFastQC Report
Fri 31 Oct 2014
AH6704C-141013_GTTTCG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6704C-141013_GTTTCG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5163515
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC946691.8334216129903758TruSeq Adapter, Index 21 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG109800.042.7628411
GATCGGA112350.042.3632131
ACACGTC110900.042.339513
CACACGT111350.042.188612
ACGTCTG111300.042.0862715
CACGTCT111350.042.04716514
AGTCACG110150.042.03911628
CGTCTGA111600.042.0134516
CGATCTC108600.041.78965438
AGCACAC112950.041.66984610
GTCACGT110900.041.62985229
CGTTTCG109550.041.60848633
GATCTCG110000.041.42383639
AGAGCAC114700.041.1705868
GAGCACA115100.041.0470549
GAACTCC113300.041.03039621
CAGTCAC113350.040.9924527
TCGGAAG116150.040.9862943
ATCTCGT112150.040.5533840
GTATGCC112000.040.55055645
Produced by FastQC (version 0.11.2)