FastQCFastQC Report
Fri 31 Oct 2014
AH6704B-141013_GTGGCC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6704B-141013_GTGGCC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7169781
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC992761.3846448029584169TruSeq Adapter, Index 20 (98% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA83080.11587522687234102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG115650.042.42926811
CACACGT117550.041.68663412
GATCGGA119450.041.676171
ACACGTC118300.041.3843113
AGTCACG116850.041.32309728
ACGTCTG118550.041.27806515
CGTGGCC115500.041.2760833
CACGTGG116550.041.23353631
CACGTCT119600.040.8968614
CGTCTGA119700.040.8814916
AGCACAC120750.040.71090310
ACGTGGC117600.040.65379332
GTCACGT118650.040.5611929
GAACTCC120650.040.37592321
GAGCACA122750.040.2305959
CATCTCG118850.040.11879339
AGAGCAC123350.040.0896228
CAGTCAC120450.040.03199427
TCCAGTC120850.040.01120825
GTATGCC119600.039.91152245
Produced by FastQC (version 0.11.2)