FastQCFastQC Report
Fri 31 Oct 2014
AH6704A-141013_GTAGAG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6704A-141013_GTAGAG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6051253
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC663991.0972768780284017TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG76150.040.44622411
ACACGTC77000.040.05817813
CACGTAG75550.039.75659631
GATCGGA79100.039.550131
AGTCACG76300.039.48636628
CGTAGAG75450.039.3908433
CACACGT78150.039.38234312
CACGTCT78050.039.317514
ACGTCTG78050.039.28867715
AGCACAC78550.039.23908210
CGTCTGA78950.038.8123116
GATCTCG76650.038.7805639
GTATGCC77350.038.5232545
GTCACGT78100.038.4306729
GAGCACA80700.038.3051959
CAGTCAC79200.038.23939527
AGAGCAC81750.037.8682448
ACGTAGA79200.037.8098132
TCGGAAG83050.037.600873
TCCAGTC80850.037.5146625
Produced by FastQC (version 0.11.2)