FastQCFastQC Report
Fri 31 Oct 2014
AH6408I-141013_CCGTCC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6408I-141013_CCGTCC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94010393
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG9587111.0197925669771426TruSeq Adapter, Index 16 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA4000970.42558805173806685No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC3101810.3299433074383595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG1266950.043.17400735
GTCCCGA1202400.042.647636
GCACACG1365500.042.5809511
ACCCGTC1341500.042.12148332
ACACGTC1385100.041.99785213
TCACCCG1353850.041.9184130
CACACGT1391350.041.8448212
CACCCGT1355600.041.7879331
ACGTCTG1390200.041.7628615
CACGTCT1392100.041.72687514
CCCGTCC1346350.041.5920833
AGCACAC1409900.041.3818410
CGTCTGA1418200.040.98111716
GAGCACA1430000.040.8159149
TCCCGAT1229900.040.6982737
GATCGGA1450000.040.6798321
CCCGATC1212600.040.67087638
CCGATCT1211250.040.5673339
CGATCTC1211200.040.5583340
CAGTCAC1414500.040.3945327
Produced by FastQC (version 0.11.2)