FastQCFastQC Report
Fri 31 Oct 2014
AH6408I-141013_CCGTCC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6408I-141013_CCGTCC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50673252
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG2857700.5639464386457771TruSeq Adapter, Index 16 (97% over 40bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC1029250.20311504775734546No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA589360.1163059359205918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG587150.042.77133635
GTCCCGA543600.042.344636
GCACACG723750.042.05401211
ACGTCTG746300.041.55179215
TCACCCG615800.041.4280930
ACCCGTC616250.041.1313632
CACGTCT748200.041.08871514
CGTCTGA753150.040.86319416
GATCGGA770350.040.734491
CCCGTCC610400.040.68148433
CACCCGT629050.040.61277831
CCCGATC556050.040.37838
GAGCACA753350.040.2458889
AGCACAC747800.040.22313310
TCGGAAG774750.040.2203143
TCCCGAT572250.040.20617337
ACACGTC758350.040.2005513
CTCGGGG221400.040.1198216
GATCTCG624300.039.97908441
CACACGT758550.039.94673512
Produced by FastQC (version 0.11.2)