FastQCFastQC Report
Fri 31 Oct 2014
AH6408A-141013_GTCCGC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6408A-141013_GTCCGC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19356278
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG2311941.194413512763146TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG275200.042.33273711
GATCGGA286300.041.7942851
ACGTCCG270650.041.75579532
CGTCCGC269650.041.65199333
ACACGTC283400.041.0602313
CACACGT284900.040.84404812
AGTCACG281050.040.75617628
GTCCGCA274800.040.74858534
ACGTCTG286450.040.5916215
CACGTCC279250.040.53430631
CCGCACA275950.040.5053936
CACGTCT287300.040.45586414
CGTCTGA287000.040.44317216
AGCACAC289450.040.3963310
GAGCACA293450.039.953039
GTCACGT284200.039.9234129
TCGGAAG302300.039.654883
TCACGTC286500.039.5399830
AGAGCAC297500.039.5302358
TCCGCAC282000.039.4608735
Produced by FastQC (version 0.11.2)