FastQCFastQC Report
Fri 31 Oct 2014
AH6408A-141013_GTCCGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6408A-141013_GTCCGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10977901
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG573360.5222856354780391TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA147750.041.562421
GCACACG140000.041.5101111
CGTCCGC120750.041.21539333
ACGTCCG123300.041.0381632
ACACGTC143200.040.708213
ACGTCTG147050.040.514515
CACACGT143600.040.32845712
CGTCTGA146450.040.15815416
TCGGAAG151900.040.0801623
AGTCACG131250.039.976328
CACGTCT147550.039.96550814
CACGTCC125950.039.7459931
GTCCGCA121800.039.45621534
CCGCACA112350.039.43048536
GTCACGT137300.039.41086229
AGCACAC145100.039.15177510
GAGCACA146950.039.0875749
AGAGCAC147250.039.0079428
TCACGTC134000.038.75167530
CATCTCG129750.038.71249441
Produced by FastQC (version 0.11.2)