FastQCFastQC Report
Fri 31 Oct 2014
AH6179F-141013_GGCTAC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6179F-141013_GGCTAC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5659119
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC240070.42421797456459215TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG30350.039.5872111
CACGGCT29850.037.9120131
GTCACGG30450.037.75640529
GATCGGA32200.037.3892751
ACACGTC32250.037.11639413
CACACGT32400.037.0834512
CGGCTAC30250.037.03816633
AGTCACG31300.037.01957328
ACGGCTA30850.036.828332
TCACGGC30950.036.63726830
AGCACAC33200.036.3234910
ACGTCTG33550.035.67820415
CACGTCT33650.035.50531414
CATCTCG32150.035.0663539
GAGCACA34600.034.9172369
CGTCTGA35100.033.97446416
CAGTCAC34450.033.6349227
GAACTCC34750.033.6035321
GGCTACA33350.033.59533734
AGAGCAC36450.033.453648
Produced by FastQC (version 0.11.2)