FastQCFastQC Report
Fri 31 Oct 2014
AH6179E-141013_TAGCTT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6179E-141013_TAGCTT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12900135
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC802840.6223500761813733TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG101700.039.26727311
ACACGTC103250.038.63540613
GATCGGA104850.038.443561
CACACGT105250.038.00812512
ACGTCTG104700.037.88559315
CACGTCT105800.037.6405614
AGCACAC107400.037.39259310
CGTCTGA107450.036.8531616
CACTAGC105150.036.73832731
TATCTCG105000.036.73108739
GAGCACA109900.036.520819
GTATGCC108000.036.278145
GAACTCC109250.036.16583621
CGTATGC110800.035.2394644
CAGTCAC110750.035.20899227
AGAGCAC114350.035.1782768
TCCAGTC111900.035.06833625
ACTAGCT110600.034.98860532
CTCGTAT111700.034.9152342
CCAGTCA112750.034.8239226
Produced by FastQC (version 0.11.2)