FastQCFastQC Report
Fri 31 Oct 2014
AH6179D-141013_GATCAG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6179D-141013_GATCAG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7443118
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC2027002.723321059803163TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG227500.043.15757411
CACACGT229400.042.68300212
ACGTCTG229500.042.65460215
ACACGTC229450.042.64428313
GATCGGA234150.042.5480731
AGTCACG227450.042.4786428
CACGTCT230400.042.4489214
CGTCTGA230850.042.38566616
CGATCAG225400.042.38297333
AGCACAC232750.042.30946710
GTCACGA227300.042.30698429
GAGCACA234700.042.072419
GATCTCG228350.042.0555939
GTATGCC229400.041.9973845
GAACTCC232100.041.85998521
CACGATC228750.041.79178631
AGAGCAC237000.041.7210738
TCCAGTC231950.041.6356825
CAGTCAC232700.041.60785327
TCGGAAG239900.041.516833
Produced by FastQC (version 0.11.2)