FastQCFastQC Report
Fri 31 Oct 2014
AH6179C-141013_ACTTGA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6179C-141013_ACTTGA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6869794
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC384260.5593471943991334TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG46300.040.0411611
ACACGTC48350.038.67002513
GATCGGA49850.037.738421
CACACGT49400.037.5292712
CACGTCT49800.037.45372814
ACGTCTG50350.036.99991615
CGTCTGA50600.036.77264416
GTCACAC49200.036.53830329
GAACTCC51400.036.1143421
AGCACAC52300.035.6190810
GAGCACA52800.035.281019
CAGTCAC51850.035.23728627
CCAGTCA53000.034.72742526
AGAGCAC53900.034.602738
CTCGTAT52050.034.45997242
CGTATGC52350.034.39146444
TCCAGTC53350.034.37307425
GTATGCC52400.034.14390645
TCGGAAG55650.033.9188733
AGTCACA54600.033.4622728
Produced by FastQC (version 0.11.2)