FastQCFastQC Report
Fri 31 Oct 2014
AH6179B-141013_TTAGGC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6179B-141013_TTAGGC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7332710
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1038821.4166931461901535TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC113650.042.0869233
ACGTCTG116650.041.7950215
GCACACG117000.041.70816811
ACACGTC117400.041.5280213
CACACGT117850.041.35035712
ACTTAGG116650.041.25526432
CACGTCT118150.041.24536514
CATCTCG116250.040.95767639
GATCGGA119800.040.9337161
CGTCTGA119450.040.83415216
GAACTCC119100.040.807221
AGCACAC120350.040.6030110
GTATGCC119400.040.24370645
CCAGTCA120550.040.2046526
TCCAGTC120650.040.17132625
TAGGCAT119200.040.12734235
CACTTAG120550.040.0512331
GAGCACA122150.040.0228279
CAGTCAC121100.039.98489427
CTCGTAT120150.039.93630642
Produced by FastQC (version 0.11.2)