FastQCFastQC Report
Fri 31 Oct 2014
AH6179A-141013_ATCACG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6179A-141013_ATCACG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6289317
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC348230.5536849231800528TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG45200.039.0728911
GATCGGA47400.037.6008951
ACACGTC46900.037.46561413
CACACGT47250.037.28332512
CGATCTC45400.037.1713638
ACGTCTG47050.037.1070815
CGTCTGA47250.036.80716316
CACGTCT47650.036.73426414
CACGATC46100.036.60285636
GATCTCG47050.036.44399639
TCCAGTC47950.036.132825
AGCACAC49500.035.7695810
GAGCACA50250.035.4592869
CATCACG48550.034.98745733
GTATGCC49250.034.9572845
GAACTCC49700.034.6791521
AGAGCAC51600.034.5751728
CCAGTCA49800.034.51942426
CGTATGC50450.034.1257944
CAGTCAC50000.034.1113427
Produced by FastQC (version 0.11.2)