FastQCFastQC Report
Fri 31 Oct 2014
AH5187A-141013_GTCCGC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH5187A-141013_GTCCGC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7986263
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG121330.1519233714191481TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC16750.033.17633433
ACGTCCG17650.031.99469632
GCACACG20600.029.5979711
AGTCACG19600.029.50378228
GATCGGA22250.028.3186261
ACGTCTG22850.026.68434515
CACGTCC21350.026.55599231
ACACGTC22750.026.50477613
CCGCACA21150.026.48715236
GTCCGCA21250.026.46837834
CACACGT23150.026.14412
GTCACGT22300.025.72848529
CACGTCT23650.025.68656714
TCACGTC22900.024.75868830
CATCTCG23200.024.73535341
CGTCTGA24800.024.40473216
TCCGCAC23200.023.95274235
CTCGGGG5850.023.46104816
AGCACAC26600.023.42922810
GAGCACA27000.023.414429
Produced by FastQC (version 0.11.2)