FastQCFastQC Report
Fri 31 Oct 2014
AH119D-141013_AGTTCC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH119D-141013_AGTTCC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7945391
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG119699915.06532529361991TruSeq Adapter, Index 14 (97% over 44bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA212030.2668591136672821No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC186150.23428677078321256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1312050.044.822711
TCGGAAG1311600.044.7384953
ATCGGAA1316550.044.657442
GCACACG1302450.044.63099711
GAGCACA1304800.044.6196259
AGCACAC1305700.044.5828210
CACACGT1305550.044.56177512
ACACGTC1305550.044.54626513
CGGAAGA1316500.044.508474
CACGTCT1304000.044.50776714
ACGTCTG1304600.044.47522715
GTCTGAA1307900.044.2899217
TCGTATG1281650.044.28151745
TCTGAAC1306850.044.26641518
GAACTCC1304500.044.206321
TCCAGTC1298100.044.16653425
TGAACTC1308200.044.16104520
AGAGCAC1320250.044.138378
ACTCCAG1302850.044.08149723
CCAGTCA1300750.044.04022626
Produced by FastQC (version 0.11.2)