FastQCFastQC Report
Fri 31 Oct 2014
AH119C-141013_CAGATC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH119C-141013_CAGATC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10177680
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC111270.10932746952154125TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG23450.028.01509511
GATCGGA27500.025.1229631
CACACGT27300.024.39437312
CGTCTGA28900.022.57682816
ACGTCTG29600.022.49897615
ACACGTC29400.022.26928513
AGCACAC31450.021.17467510
CATCTCG26800.020.82250439
CACGTCT32400.020.27685514
GAACTCC32350.020.10112821
GAGCACA33100.019.98319
CTCGGGG6000.019.87409616
CAGTCAC33700.019.09558727
AGAGCAC35500.018.885638
TCCAGTC35100.018.78267325
GTATGCC31600.018.16099445
CGTATGC31450.018.10449444
CCAGTCA36650.017.80413626
TCGGAAG38800.017.80153
AGTCACC36500.017.5071728
Produced by FastQC (version 0.11.2)