FastQCFastQC Report
Fri 31 Oct 2014
AH119B-141013_CTTGTA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH119B-141013_CTTGTA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8152555
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1480601.8161177692146817TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG164800.043.03009411
CACACGT167750.042.28756712
ACACGTC168250.042.1485313
AGCACAC168950.042.03970710
ACGTCTG168850.041.97262215
GATCGGA172100.041.8044241
CACGTCT170450.041.57862514
CGTCTGA170850.041.4944516
GTATGCC168300.041.46796845
GAGCACA171800.041.4601639
GAACTCC170500.041.34438721
AGAGCAC175450.040.674588
TCGGAAG177050.040.6119773
TCCAGTC173050.040.5533725
CTCGTAT172150.040.51442342
CGTATGC172800.040.47924444
CAGTCAC173300.040.46890627
GTCTGAA176500.040.26813517
TCTCGTA173650.040.16298341
AGTCACC174500.040.1256528
Produced by FastQC (version 0.11.2)