FastQCFastQC Report
Fri 31 Oct 2014
AH119A-141013_AGTCAA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH119A-141013_AGTCAA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10370726
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG333060.32115398671221285TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG43800.036.67580811
GATCGGA46950.034.7043721
ACACGTC47000.034.1315213
ACGTCTG47350.033.92690715
CACACGT47900.033.678112
CGTCTGA48850.033.16148816
CACGTCT49450.032.62262714
AGCACAC50750.031.74156610
GAGCACA52500.031.068759
AGAGCAC53700.030.458278
CAGTCAC52750.030.24245527
CACAGTC51150.030.17451731
TCCAGTC53400.029.91647125
GAACTCC53300.029.84566921
CCAGTCA53500.029.7343826
GTCACAG53200.029.60442529
AGTCACA55000.028.92316428
ATCTCGT54600.028.8935142
CTCGTAT54950.028.7504344
TCGGAAG57650.028.1763733
Produced by FastQC (version 0.11.2)