##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename Undetermined_Undetermined_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24604663 Filtered Sequences 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.181508318159043 31.0 28.0 31.0 16.0 34.0 2 29.99651338447513 31.0 30.0 31.0 26.0 34.0 3 29.98719096457448 31.0 30.0 31.0 26.0 34.0 4 33.002938101611065 35.0 33.0 35.0 26.0 37.0 5 32.80467588603022 35.0 32.0 35.0 26.0 37.0 6 33.34166084697035 35.0 33.0 35.0 28.0 37.0 7 32.80536299155977 35.0 32.0 35.0 26.0 37.0 8 32.30846961813702 35.0 32.0 35.0 25.0 37.0 9 33.67291935679022 35.0 32.0 38.0 25.0 39.0 10 33.91592837504013 35.0 32.0 39.0 27.0 39.0 11 34.28353877474363 37.0 33.0 39.0 27.0 39.0 12 33.2204711358981 35.0 32.0 38.0 25.0 39.0 13 32.91409677100637 35.0 32.0 38.0 25.0 39.0 14 34.32660508294708 36.0 32.0 39.0 25.0 40.0 15 34.20730911860081 36.0 32.0 39.0 25.0 40.0 16 34.049279154930915 36.0 32.0 39.0 25.0 40.0 17 34.501436902427805 36.0 32.0 39.0 27.0 40.0 18 34.83393599010074 37.0 33.0 39.0 27.0 40.0 19 34.6230759998623 37.0 32.0 39.0 26.0 40.0 20 34.949079895953055 37.0 33.0 39.0 27.0 40.0 21 34.67273122172004 37.0 32.0 39.0 26.0 40.0 22 34.56405308213325 37.0 32.0 39.0 26.0 40.0 23 34.2425127302089 37.0 32.0 39.0 25.0 40.0 24 34.28706387890783 37.0 32.0 39.0 25.0 40.0 25 34.04835770357838 37.0 32.0 39.0 25.0 40.0 26 33.694396871032126 37.0 32.0 39.0 24.0 40.0 27 32.67819900642411 36.0 30.0 39.0 18.0 40.0 28 32.46990832591367 36.0 30.0 39.0 17.0 40.0 29 32.663606975637094 36.0 30.0 39.0 18.0 40.0 30 32.57668117624696 36.0 30.0 39.0 18.0 40.0 31 32.29854105297032 36.0 30.0 39.0 17.0 40.0 32 32.71323029297333 36.0 30.0 39.0 18.0 40.0 33 32.039093321456996 36.0 30.0 38.0 16.0 40.0 34 32.97516576431061 36.0 30.0 39.0 21.0 40.0 35 33.02657016680131 36.0 30.0 39.0 19.0 40.0 36 32.99747478760428 36.0 30.0 39.0 19.0 40.0 37 32.93763454512667 36.0 30.0 39.0 19.0 40.0 38 33.522267506773005 36.0 31.0 39.0 23.0 40.0 39 32.451341845242915 36.0 30.0 39.0 16.0 40.0 40 32.93942221439895 36.0 30.0 39.0 19.0 40.0 41 32.553059109161545 36.0 30.0 39.0 18.0 40.0 42 31.059669380556034 35.0 27.0 38.0 11.0 40.0 43 31.691519286405182 35.0 29.0 38.0 15.0 40.0 44 31.71207071602647 35.0 29.0 38.0 15.0 40.0 45 31.901410029472867 35.0 30.0 38.0 15.0 40.0 46 32.28160629552211 35.0 30.0 39.0 18.0 40.0 47 31.93474789717705 35.0 30.0 39.0 15.0 40.0 48 31.203929393383685 35.0 27.0 38.0 13.0 40.0 49 31.52791716757104 35.0 28.0 38.0 15.0 40.0 50 31.20331845227874 35.0 28.0 38.0 14.0 40.0 51 30.5634609992423 34.0 26.0 38.0 13.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 14.0 10 69.0 11 177.0 12 747.0 13 2537.0 14 7180.0 15 16014.0 16 28187.0 17 40904.0 18 49830.0 19 57755.0 20 77711.0 21 120883.0 22 182751.0 23 263459.0 24 372675.0 25 508546.0 26 676327.0 27 872338.0 28 1099655.0 29 1344046.0 30 1598657.0 31 1846614.0 32 2075651.0 33 2259954.0 34 2397316.0 35 2442768.0 36 2330870.0 37 2004300.0 38 1435588.0 39 491132.0 40 6.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.684505007961057 25.32611539607757 30.745386346349374 25.243993249611997 2 20.887113958845934 27.66893007232003 28.869816262063818 22.574139706770215 3 22.278269022146883 27.121892801015225 29.005013526719107 21.59482465011878 4 22.22094649294729 26.20052548575853 29.44381721464748 22.13471080664669 5 21.505806414955707 27.656669198849304 28.827446350777457 22.01007803541753 6 21.89350449546901 28.201243804883653 27.394847879038213 22.510403820609127 7 21.693741548095986 27.99318974618754 28.468128175541356 21.844940530175112 8 21.623776066090883 28.002232599169158 27.96262729759275 22.411364037147212 9 21.49743729471117 27.341630324300723 28.5320550824045 22.628877298583603 10 21.9228038197475 27.75939666395756 27.50727372287115 22.810525793423793 11 21.702479729147274 27.599231901692782 27.883202464508454 22.81508590465149 12 21.06274733370662 27.513272585769617 28.151923885322063 23.2720561952017 13 21.387056799222076 27.79566433143424 28.034754622438534 22.78252424690515 14 21.613859522609886 27.799798225189647 27.776152295071622 22.81018995712885 15 21.454532567098237 27.80558441981232 27.841077735237914 22.898805277851533 16 21.451178668213476 27.70289125211129 27.608750449204 23.237179630471235 17 21.9288044717691 27.627175741313515 27.738928459865956 22.705091327051427 18 21.86461159821616 27.683658987729277 27.679911730552863 22.7718176835017 19 21.798960821474758 27.42167560597444 27.842828098958783 22.93653547359202 20 22.0214016804168 27.431257800875525 27.759354852606887 22.78798566610078 21 22.14113260164895 27.263028989972902 27.77757522985497 22.81826317852318 22 21.81844200940308 27.55350064675734 27.81547130698632 22.81258603685326 23 21.568199093782333 27.618138042242414 27.88089418050105 22.932768683474205 24 21.71585647383745 27.504285062783033 27.781452104778374 22.998406358601137 25 21.84956530185824 27.39966745825853 27.946475492421484 22.80429174746174 26 22.172966477706723 27.59126225909037 27.467998735863496 22.767772527339403 27 21.897055466695196 27.498925032391945 27.430278193041723 23.173741307871133 28 22.090160703673373 27.45794522310424 27.74727718677692 22.704616886445464 29 22.345563213164418 27.291753205145003 27.64429376739505 22.718389814295524 30 22.345483210921604 27.189678038492183 27.57582557588143 22.889013174704782 31 22.22029270925017 27.341516391949934 27.341299985251677 23.09689091354822 32 22.178467394099144 27.518471627259178 27.637081059127315 22.665979919514356 33 22.01315547266511 27.148659062951424 27.816863887937142 23.021321576446322 34 22.269701738294803 27.49138579278293 27.282618403719294 22.95629406520297 35 22.13325551815883 27.594308997451716 27.63274957383897 22.639685910550483 36 22.351244367666602 27.583406001904503 27.328747713988434 22.736601916440463 37 22.05796545267093 27.549095476814184 27.80021404109414 22.592725029420745 38 22.420216903603336 27.508148102632756 27.50367857581756 22.567956417946352 39 22.247105590934957 27.190099795376383 27.810224971598206 22.752569642090453 40 22.31552976137661 27.50291802232355 27.44197576133707 22.73957645496278 41 22.134927841622346 27.281425333519916 27.993920494531814 22.589726330325927 42 21.398469501021303 27.00229360903712 28.843038706968855 22.756198182972724 43 21.88602977604127 27.308725963380066 28.291937996373758 22.513306264204907 44 22.076418112666982 27.301333070749195 27.768893544945094 22.85335527163873 45 22.211813207147788 27.32631848095718 27.878444956089698 22.583423355805333 46 22.256495695689804 27.44275426814467 27.694764621700457 22.605985414465067 47 21.999290738382697 27.528948951346603 27.77381132995379 22.697948980316905 48 21.842683810218134 27.151495571364563 28.45153941341253 22.554281205004777 49 22.222876402329373 27.330450918630262 27.8957156464569 22.55095703258347 50 21.900586054714186 27.194820141674093 28.108812789489505 22.795781014122213 51 22.222232667296367 27.15778769514861 27.63123293337328 22.98874670418174 >>END_MODULE >>Per base GC content pass #Base %GC 1 43.928498257573054 2 43.46125366561615 3 43.87309367226567 4 44.35565729959398 5 43.515884450373235 6 44.40390831607813 7 43.538682078271094 8 44.0351401032381 9 44.12631459329477 10 44.73332961317129 11 44.51756563379876 12 44.334803528908324 13 44.16958104612723 14 44.424049479738734 15 44.35333784494977 16 44.68835829868471 17 44.633895798820525 18 44.63642928171786 19 44.73549629506678 20 44.80938734651758 21 44.95939578017212 22 44.63102804625634 23 44.50096777725654 24 44.71426283243859 25 44.65385704931998 26 44.940739005046126 27 45.07079677456633 28 44.79477759011884 29 45.06395302745994 30 45.234496385626386 31 45.31718362279839 32 44.8444473136135 33 45.03447704911143 34 45.225995803497774 35 44.77294142870931 36 45.087846284107066 37 44.650690482091676 38 44.98817332154969 39 44.99967523302541 40 45.05510621633939 41 44.724654171948266 42 44.15466768399403 43 44.399336040246176 44 44.92977338430571 45 44.79523656295312 46 44.862481110154874 47 44.6972397186996 48 44.39696501522291 49 44.77383343491284 50 44.6963670688364 51 45.21097937147811 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 160.0 1 237.5 2 315.0 3 482.0 4 649.0 5 1090.5 6 1532.0 7 2415.0 8 3298.0 9 5019.5 10 6741.0 11 10081.0 12 13421.0 13 19413.0 14 25405.0 15 34791.5 16 44178.0 17 59388.0 18 74598.0 19 96701.0 20 118804.0 21 150522.5 22 182241.0 23 226309.5 24 270378.0 25 327259.5 26 461619.0 27 539097.0 28 641591.0 29 744085.0 30 870161.5 31 996238.0 32 1130480.5 33 1264723.0 34 1385838.5 35 1506954.0 36 1573597.0 37 1640240.0 38 1666170.5 39 1692101.0 40 1676736.5 41 1661372.0 42 1652817.0 43 1644262.0 44 1621747.5 45 1599233.0 46 1567246.0 47 1535259.0 48 1492305.5 49 1449352.0 50 1384166.0 51 1318980.0 52 1242515.0 53 1166050.0 54 1057930.5 55 949811.0 56 856248.0 57 762685.0 58 682954.0 59 603223.0 60 538896.5 61 474570.0 62 424753.0 63 374936.0 64 338395.5 65 301855.0 66 276159.5 67 250464.0 68 229915.0 69 209366.0 70 194097.0 71 178828.0 72 165371.0 73 151914.0 74 139181.0 75 113714.0 76 100980.0 77 89665.0 78 78350.0 79 67724.0 80 57098.0 81 48406.0 82 39714.0 83 32907.0 84 26100.0 85 21117.5 86 16135.0 87 12712.0 88 9289.0 89 7111.5 90 4934.0 91 3699.0 92 2464.0 93 1787.0 94 1110.0 95 766.0 96 422.0 97 275.5 98 129.0 99 80.0 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.340296227589055 2 0.0 3 1.0973529692318891E-4 4 0.0 5 2.6824183692335067E-4 6 0.0 7 0.0 8 1.5880445100995695 9 0.0 10 0.0 11 0.0 12 0.0 13 1.904488592263995 14 2.4385621538486424E-5 15 1.2192810769243212E-4 16 1.1786383743601771E-4 17 0.12867073204782362 18 0.0 19 1.4631372923091854E-4 20 2.154063235899634E-4 21 0.005820035007185427 22 0.0015484869676938879 23 0.003499336690772802 24 2.4385621538486424E-5 25 9.469749697445561E-4 26 0.5261522988548959 27 0.4696386209394537 28 0.49467452571896636 29 0.747529035451532 30 0.45816112173533935 31 0.4622660346943179 32 0.5909205096611159 33 0.48935439595331987 34 0.47990903187741285 35 0.49876724586717563 36 0.5072209280005177 37 0.512854006575908 38 0.5194096744995045 39 0.510362608908726 40 0.5111023060953934 41 0.5242420918343812 42 0.645853186446813 43 0.5295256431677199 44 0.5282250766856672 45 0.5593411297687759 46 0.5439741239292731 47 0.5337362271533652 48 0.5672298783364763 49 0.5266562683666913 50 0.5428605138790155 51 0.5610847017087777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2.4604663E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 6.019455927126916 #Duplication Level Relative count 1 100.0 2 1.7857519121768264 3 0.5757601297962451 4 0.4298447080638024 5 0.36083777576434395 6 0.280241893002381 7 0.22809161592111085 8 0.1801555026443878 9 0.14064771697675887 10++ 1.3727638593312121 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content pass >>END_MODULE