##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	16634007	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.967029651965397	31.0	28.0	31.0	16.0	34.0
2	29.85648647376426	31.0	30.0	31.0	26.0	34.0
3	30.319650641003097	31.0	30.0	31.0	27.0	34.0
4	33.342641313064256	35.0	33.0	35.0	28.0	37.0
5	31.21135935556598	35.0	32.0	35.0	19.0	37.0
6	32.077099883389494	35.0	32.0	35.0	18.0	37.0
7	31.802807826159988	35.0	32.0	35.0	25.0	37.0
8	32.25239552923117	35.0	32.0	35.0	25.0	37.0
9	33.144124864201395	35.0	32.0	38.0	25.0	39.0
10	33.308414623127184	35.0	32.0	38.0	25.0	39.0
11	34.24194471001485	37.0	33.0	39.0	27.0	39.0
12	33.69064802004712	35.0	32.0	39.0	26.0	39.0
13	33.92975198339161	37.0	32.0	39.0	26.0	39.0
14	34.8294659849548	37.0	33.0	39.0	27.0	40.0
15	33.65905154422503	36.0	32.0	39.0	24.0	40.0
16	34.7223762740992	37.0	32.0	39.0	27.0	40.0
17	34.94800903955373	37.0	33.0	39.0	27.0	40.0
18	34.78249474104466	37.0	33.0	39.0	27.0	40.0
19	34.895813137508	37.0	33.0	40.0	27.0	40.0
20	34.66443485324973	37.0	32.0	39.0	26.0	40.0
21	34.80608484774594	37.0	33.0	39.0	27.0	40.0
22	34.96195468716588	37.0	33.0	39.0	27.0	40.0
23	34.50251193233236	37.0	32.0	39.0	25.0	40.0
24	34.768422425216	37.0	33.0	39.0	27.0	40.0
25	35.11391368297488	37.0	33.0	39.0	27.0	40.0
26	33.086785883882335	36.0	31.0	39.0	18.0	40.0
27	33.20421321212622	36.0	31.0	39.0	19.0	40.0
28	33.24217658439124	36.0	31.0	39.0	20.0	40.0
29	33.4329611620339	36.0	31.0	39.0	23.0	40.0
30	32.42383311489529	36.0	30.0	39.0	17.0	40.0
31	32.846750034432475	36.0	30.0	39.0	18.0	40.0
32	33.03171454719239	36.0	30.0	39.0	19.0	40.0
33	32.91749708894556	36.0	30.0	39.0	19.0	40.0
34	33.638965103237	37.0	31.0	39.0	24.0	40.0
35	32.8547662027556	36.0	30.0	39.0	18.0	40.0
36	32.83909379141178	36.0	30.0	39.0	16.0	40.0
37	33.065322985616156	36.0	30.0	39.0	19.0	40.0
38	31.405539687460756	35.0	28.0	39.0	12.0	40.0
39	31.709961165701085	35.0	29.0	39.0	15.0	40.0
40	32.18191846378326	35.0	30.0	39.0	16.0	40.0
41	32.77160668502785	36.0	30.0	39.0	19.0	40.0
42	32.97957449458811	36.0	30.0	39.0	19.0	40.0
43	32.59492640588645	36.0	30.0	39.0	18.0	40.0
44	32.90985172724768	36.0	30.0	39.0	19.0	40.0
45	32.08411370753902	35.0	30.0	39.0	15.0	40.0
46	31.419177712261394	35.0	28.0	39.0	14.0	40.0
47	32.18077838971692	35.0	30.0	39.0	16.0	40.0
48	32.214167999328126	35.0	30.0	39.0	15.0	40.0
49	32.52681702009624	36.0	30.0	39.0	18.0	40.0
50	32.619470161338754	36.0	30.0	39.0	19.0	40.0
51	31.319426401588025	35.0	28.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	1.0
8	1.0
9	15.0
10	54.0
11	213.0
12	862.0
13	3025.0
14	8519.0
15	19748.0
16	34851.0
17	48081.0
18	52603.0
19	49140.0
20	50864.0
21	78178.0
22	119617.0
23	174875.0
24	246093.0
25	333286.0
26	437359.0
27	560495.0
28	698024.0
29	846593.0
30	1003494.0
31	1159474.0
32	1309193.0
33	1444842.0
34	1566070.0
35	1650041.0
36	1654957.0
37	1489391.0
38	1130740.0
39	463294.0
40	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.421590846226245	24.761900610132468	31.706847592014903	25.109660951626385
2	20.81161803046013	27.58818725999093	28.84537081173526	22.75482389781368
3	21.685514500504897	27.352393202672094	29.483214717896892	21.478877578926113
4	21.62979130644829	26.036763120275232	30.226883997343513	22.106561575932968
5	21.472149117136954	27.651993018763317	29.205786891420065	21.670070972679664
6	21.761144008244315	28.15197373380772	27.35260525937047	22.734276998577492
7	21.77670731676328	28.132863298449696	28.678643883374512	21.411785501412517
8	21.22839072990651	28.32996282855959	28.296585422862936	22.145061018670965
9	21.169252919908477	27.202505323523518	29.07424963016329	22.55399212640472
10	21.63699171587943	27.785636468912017	27.719065549419902	22.85830626578865
11	21.670172436503123	27.611969863905912	28.021299979012877	22.696557720578088
12	21.55502444406083	27.670618175309663	27.759107778331465	23.01524960229804
13	21.39960022861599	28.076355865426773	27.984561987980406	22.53948191797683
14	21.458190314942353	27.834222970598717	27.882058921362734	22.8255277930962
15	20.788508745968425	27.84240141296081	28.713616628873606	22.65547321219716
16	21.603734337557277	27.776308875815058	27.830247436836213	22.789709349791448
17	21.889181602484598	27.71337657847565	27.849856020861356	22.547585798178392
18	20.92734131076671	27.638046279293533	28.798482385931713	22.63613002400805
19	21.808842438278692	27.577401273683172	27.80631243400551	22.807443854032627
20	21.8359150026993	27.369101968625593	28.14657260997695	22.648410418698155
21	21.955966033262353	27.370348232288887	28.04052888183944	22.633156852609314
22	21.777288509008873	27.840394280461183	27.863774893454085	22.518542317075863
23	21.582050338402585	27.971893889850314	27.902353717774766	22.543702053972336
24	21.753236954819883	27.609724372937883	27.85016566672666	22.78687300551557
25	21.88365551050168	27.58305442520076	28.187404141611427	22.345885922686136
26	21.81347517596931	27.480828104155826	27.923124453584336	22.78257226629053
27	22.051414440774867	27.52510069739514	27.46927142526525	22.954213436564743
28	22.21978029053763	27.88497675639429	27.407457622918002	22.487785330150075
29	22.41068767597829	27.469257113425783	27.685162191936918	22.434893018659004
30	21.91592993803152	27.21231626729746	28.27842040910358	22.593333385567437
31	22.042767150289322	27.53901287198468	27.50609210195173	22.912127875774267
32	21.934740278926483	27.773062722102427	27.908515868810348	22.383681130160745
33	21.93756258023038	27.494963367544113	27.68713671430401	22.880337337921492
34	22.039784355521817	27.685502918970773	27.633287759469134	22.64142496603828
35	22.16892568000063	27.783011738999875	27.57585800195979	22.4722045790397
36	22.084065433125208	27.796445069785058	27.528816777932473	22.590672719157258
37	21.899041689630426	27.801628355924407	27.742359068340754	22.55697088610441
38	21.692524961921052	27.18850242321682	28.611839379298875	22.507133235563252
39	22.027333063343864	27.323237816209073	27.51472022538716	23.1347088950599
40	22.160738768652894	27.665117854728088	27.46287253003743	22.71127084658159
41	22.146481066725357	27.49538766424661	27.99099171155112	22.367139557476907
42	22.22767014039777	27.502688277349446	27.50482007141814	22.764821510834647
43	21.941152321322935	27.47638012104235	28.034475431109946	22.54799212652477
44	22.042708601262408	27.62646146102079	27.570170150572572	22.76065978714423
45	22.08613818061367	27.480291411262087	28.077581989133638	22.355988418990602
46	21.758546077214245	27.40095768475408	28.4585653544	22.381930883631675
47	22.09028987702837	27.902335872178195	27.52123264658306	22.486141604210378
48	22.01330983859063	27.428562399478086	27.943684329353026	22.614443432578263
49	22.46185451627902	27.609402987642195	27.545278832482044	22.383463663596743
50	22.191284700959596	27.533315728436243	27.599771581928824	22.675627988675345
51	22.151790338639064	27.316814267719568	27.677066261789356	22.854329131852012
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.531251797852626
2	43.56644192827381
3	43.16439207943101
4	43.736352882381254
5	43.14222008981662
6	44.49542100682181
7	43.188492818175796
8	43.37345174857748
9	43.723245046313195
10	44.495297981668074
11	44.366730157081214
12	44.57027404635887
13	43.939082146592824
14	44.28371810803855
15	43.44398195816559
16	44.39344368734873
17	44.436767400662994
18	43.56347133477475
19	44.61628629231132
20	44.48432542139746
21	44.58912288587167
22	44.29583082608473
23	44.12575239237492
24	44.54010996033545
25	44.22954143318781
26	44.59604744225984
27	45.00562787733961
28	44.7075656206877
29	44.8455806946373
30	44.509263323598965
31	44.95489502606359
32	44.31842140908723
33	44.81789991815187
34	44.681209321560104
35	44.64113025904034
36	44.674738152282465
37	44.45601257573484
38	44.199658197484304
39	45.16204195840377
40	44.87200961523448
41	44.51362062420227
42	44.99249165123242
43	44.48914444784771
44	44.80336838840664
45	44.44212659960428
46	44.14047696084592
47	44.57643148123874
48	44.6277532711689
49	44.84531817987577
50	44.866912689634944
51	45.00611947049107
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	222.0
1	273.0
2	324.0
3	605.0
4	886.0
5	1390.5
6	1895.0
7	2967.5
8	4040.0
9	5954.5
10	7869.0
11	11600.0
12	15331.0
13	21130.0
14	26929.0
15	35731.5
16	44534.0
17	57325.5
18	70117.0
19	87540.5
20	104964.0
21	128487.5
22	152011.0
23	183276.0
24	214541.0
25	254377.0
26	345147.5
27	396082.0
28	461764.0
29	527446.0
30	607070.0
31	686694.0
32	770824.0
33	854954.0
34	930448.5
35	1005943.0
36	1048384.0
37	1090825.0
38	1109192.0
39	1127559.0
40	1119606.5
41	1111654.0
42	1107341.0
43	1103028.0
44	1088363.5
45	1073699.0
46	1051362.5
47	1029026.0
48	997919.5
49	966813.0
50	924980.5
51	883148.0
52	830627.5
53	778107.0
54	702197.0
55	626287.0
56	560923.5
57	495560.0
58	441997.5
59	388435.0
60	346947.5
61	305460.0
62	271815.0
63	238170.0
64	214994.5
65	191819.0
66	175279.0
67	158739.0
68	145876.0
69	133013.0
70	123369.0
71	113725.0
72	106193.0
73	98661.0
74	90815.5
75	75664.0
76	68358.0
77	60718.5
78	53079.0
79	46232.0
80	39385.0
81	33370.5
82	27356.0
83	22743.0
84	18130.0
85	14437.0
86	10744.0
87	8280.0
88	5816.0
89	4463.5
90	3111.0
91	2256.5
92	1402.0
93	1000.5
94	599.0
95	416.5
96	234.0
97	154.5
98	75.0
99	50.0
100	25.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	2.087386400642972
2	0.0
3	0.0
4	0.0
5	5.599041770272191
6	6.011780564959484E-6
7	3.0017902481344394
8	0.0
9	0.14006246360242605
10	0.003715280389144961
11	0.0
12	1.653377926316852
13	0.0
14	4.448717618070018E-4
15	0.0
16	0.0016051454108441821
17	0.0
18	1.2023561129918968E-5
19	2.5850656429325777E-4
20	4.027892978522854E-4
21	0.006186122201343308
22	0.0034146913608969864
23	0.011386312390033262
24	5.410602508463535E-5
25	5.711191536711509E-4
26	1.0419858546410374
27	1.0299202110471637
28	1.031801898363996
29	1.2994102984326026
30	1.0200067848955456
31	0.913856775460056
32	1.1152754715084585
33	1.0108448313145475
34	1.0301486587086321
35	2.6233907440341944
36	1.0182753920928374
37	1.063586182210937
38	1.0191711473970162
39	1.0021517966176159
40	1.047817281789048
41	1.0090052264616698
42	1.0160029390392826
43	1.0380000441264694
44	1.0626423326622383
45	1.1226158555782741
46	1.0780505262502293
47	1.070686095058154
48	1.0776838076357669
49	1.1008051156886012
50	1.0608688573955753
51	1.0819341364951933
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.6634007E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	13.421375177432088
#Duplication Level	Relative count
1	100.0
2	5.047198580965011
3	1.494575100860038
4	0.7822598244112068
5	0.6507640742419409
6	0.5584372709316054
7	0.490730948504026
8	0.4084761601002725
9	0.36483076217175026
10++	2.114004017614835
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	1724525	2.5688126	6.6537223	12
GGAAG	1195440	1.9972239	6.3637576	5
TCCAG	1265860	1.9203355	5.8227687	25
CTGAA	1473190	1.8339688	5.265657	19
CTCCA	1208350	1.7642992	5.5675974	24
AGAGC	975865	1.5691942	5.5798383	8
GCACA	998105	1.5447251	5.7374263	11
GAGCA	909850	1.4630419	5.441786	9
GTCTG	888405	1.3725586	5.5074673	17
AGCAC	866240	1.3406432	5.5592866	10
ACTCC	750840	1.0962936	5.0482564	23
CGGAA	352645	0.56705433	5.1915083	4
>>END_MODULE