FastQCFastQC Report
Wed 23 Jul 2014
SV25_CAGATC_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SV25_CAGATC_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7110737
Filtered Sequences 0
Sequence length 51
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC 23240 0.3268296943059489 TruSeq Adapter, Index 7 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA 11030 0.15511753563660138 No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC 8359 0.11755462197519048 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACAC 746670 2.8640375 11.838825 12
CCAGG 472520 2.7297623 6.783471 5
CCCAG 452295 2.533338 6.870746 34
CAGGG 407460 2.427856 6.6234717 6
CCCTG 417165 2.328358 6.4997187 38
TCCAG 541880 2.1362739 10.492001 25
CTCCC 389285 2.106572 6.3039412 44
GGAAG 491285 2.0676801 10.8225155 5
CAGGC 352015 2.0336013 6.238462 36
GGGGA 327920 2.0152972 6.290072 19
CTGAA 707175 1.9692125 7.6808558 19
CCAGA 483650 1.9134384 10.753026 33
CTCCA 500030 1.9112461 10.450109 24
GCACA 434390 1.7185539 10.689144 11
AGAGC 407690 1.6635914 10.564649 8
CACCA 431600 1.6555086 10.110297 31
GAAGA 566620 1.6331286 7.8772197 6
GAGCA 388255 1.5842861 10.571347 9
AAGAG 547890 1.5791446 7.826525 7
TCTGA 545965 1.5149597 7.1180396 18
GTCTG 366660 1.4856658 9.463361 17
ACCAG 374755 1.4826229 10.259815 32
AGCAC 374020 1.4797151 10.420082 10
CCAGT 370195 1.4594337 9.666598 26
CATCT 536835 1.4442545 7.285849 39
CAGTC 340195 1.3411634 9.5952 27
CAGAT 470175 1.309258 7.5189104 34
ATGCC 318140 1.2542152 9.282432 47
TCACC 322620 1.2331384 9.697449 30
ACTCC 322315 1.2319725 9.987052 23
AACTC 435225 1.175023 7.2943945 22
AGTCA 419430 1.1679525 6.9326677 28
GTCAC 288120 1.1358662 9.584073 29
ATCTC 413795 1.1132385 7.0414457 40
TCATC 406960 1.0948502 6.97533 38
GACCC 194560 1.089745 5.3296366 42
GAACT 381460 1.0622206 6.7973266 21
TGAAC 356540 0.99282795 6.7299647 20
CACGT 246410 0.9714314 9.6038 14
ATCAT 497290 0.94498444 5.0879946 37
AGATC 329515 0.9175736 7.0488634 35
GTATG 290200 0.8305521 6.2928047 45
CGGGG 94800 0.8248362 6.9011135 18
GATCA 295925 0.8240382 6.8803177 36
TATGC 289460 0.8032021 6.3008246 46
ACGTC 187745 0.7401541 9.258811 15
GGCGG 82875 0.7210791 7.050219 38
CGGAA 148395 0.6055303 8.899575 4
TCTCG 151530 0.59528226 8.973797 41
ACACG 129745 0.51330316 9.236896 13
CGTCT 114005 0.4478661 8.960011 16
CTCGT 110545 0.4342736 8.56555 42
TCGGA 95485 0.38825965 8.806305 3
ATCGG 83720 0.34042096 8.812026 2
GATCG 82740 0.3364361 9.021382 1
CGTAT 94325 0.26173577 5.8062787 44
TCGTA 85705 0.23781674 5.7963967 43
Produced by FastQC (version 0.10.1)