##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename SV25_CAGATC_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7110737 Filtered Sequences 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.46392223478382 31.0 30.0 31.0 25.0 34.0 2 30.337014151978902 31.0 30.0 31.0 27.0 34.0 3 30.75950087874154 31.0 30.0 33.0 27.0 34.0 4 32.566045263662545 35.0 32.0 37.0 25.0 37.0 5 33.22307139189651 35.0 33.0 37.0 28.0 37.0 6 33.44215669908759 35.0 33.0 37.0 28.0 37.0 7 33.48036652178248 35.0 33.0 37.0 28.0 37.0 8 33.719670689550185 35.0 33.0 37.0 29.0 37.0 9 35.15322181090371 37.0 34.0 39.0 29.0 39.0 10 35.02519499736807 37.0 34.0 39.0 28.0 39.0 11 35.00814205334834 37.0 34.0 39.0 28.0 39.0 12 35.21161842998834 37.0 34.0 39.0 30.0 39.0 13 35.0111617122107 37.0 34.0 39.0 29.0 39.0 14 35.88613275951565 38.0 34.0 40.0 28.0 41.0 15 36.245613640330106 38.0 34.0 40.0 30.0 41.0 16 35.90444380097309 38.0 34.0 40.0 29.0 41.0 17 35.13589786825191 38.0 33.0 40.0 27.0 41.0 18 35.527641930787205 38.0 34.0 40.0 27.0 41.0 19 35.541632323063 38.0 34.0 40.0 27.0 41.0 20 35.72227660789592 38.0 34.0 40.0 27.0 41.0 21 35.23152649296409 38.0 34.0 40.0 27.0 41.0 22 35.372189549409576 38.0 34.0 40.0 27.0 41.0 23 35.599117503572415 38.0 34.0 40.0 27.0 41.0 24 35.87205615957952 38.0 34.0 40.0 29.0 41.0 25 35.94468491803311 38.0 34.0 40.0 29.0 41.0 26 35.425631407827346 38.0 34.0 40.0 27.0 41.0 27 34.95297463539996 38.0 33.0 40.0 26.0 41.0 28 34.50341771886655 37.0 33.0 40.0 24.0 40.0 29 34.356713657107555 37.0 32.0 40.0 24.0 40.0 30 34.63809321030999 37.0 33.0 40.0 25.0 40.0 31 34.704006631098856 37.0 33.0 40.0 25.0 40.0 32 34.738723145012955 37.0 33.0 40.0 25.0 40.0 33 34.573110072837736 37.0 33.0 40.0 25.0 40.0 34 35.200820252527976 38.0 34.0 40.0 27.0 40.0 35 34.77238547846728 38.0 33.0 40.0 25.0 41.0 36 35.28816647275803 38.0 34.0 40.0 27.0 41.0 37 35.00916374772404 38.0 34.0 40.0 25.0 41.0 38 35.04758775356197 38.0 34.0 40.0 25.0 41.0 39 34.45706162385137 38.0 33.0 40.0 24.0 41.0 40 34.0645610996441 37.0 32.0 40.0 24.0 41.0 41 34.48372932369739 37.0 33.0 40.0 24.0 40.0 42 34.51297706552781 37.0 33.0 40.0 24.0 40.0 43 34.33635149211678 37.0 33.0 40.0 24.0 40.0 44 34.37517236258351 37.0 33.0 40.0 24.0 40.0 45 33.978800931605264 37.0 32.0 40.0 23.0 40.0 46 33.599424082201324 37.0 31.0 40.0 21.0 40.0 47 34.12653175050631 37.0 32.0 40.0 24.0 40.0 48 33.53081769161199 37.0 31.0 40.0 21.0 40.0 49 33.618200898162875 37.0 31.0 40.0 22.0 40.0 50 33.98698644036476 37.0 32.0 40.0 24.0 40.0 51 33.1388958697249 36.0 30.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 6.0 11 22.0 12 21.0 13 31.0 14 62.0 15 203.0 16 408.0 17 1011.0 18 2106.0 19 4135.0 20 7489.0 21 12597.0 22 20825.0 23 32731.0 24 49516.0 25 72848.0 26 103956.0 27 143959.0 28 194264.0 29 255633.0 30 328043.0 31 410112.0 32 499548.0 33 594751.0 34 693631.0 35 791043.0 36 862717.0 37 871927.0 38 781569.0 39 375563.0 40 6.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.189458777827152 27.44901517514101 30.847206379470098 22.51431966756174 2 18.737143561912077 30.120365863622855 30.541869288654606 20.60062128581046 3 20.193588934592857 29.029367841898807 31.242148317396634 19.534894906111703 4 20.40621105800988 27.425145944787438 32.18490572777477 19.98373726942791 5 20.201003244251538 29.824683285023717 29.797991233250137 20.1763222374746 6 20.762728251656615 29.994274855053703 28.835885787928873 20.40711110536081 7 19.625054336843004 30.437154404670007 29.95448994949469 19.983301308992303 8 19.654924658301944 30.188122553259948 29.56517728049849 20.591775507939612 9 20.760787524556175 29.136093769183134 29.599885356468675 20.503233349792012 10 20.491673366628522 30.189542940485637 28.770983935983008 20.547799756902833 11 20.35222228019402 29.87229031252316 29.252790533526973 20.522696873755844 12 19.820463054673517 29.700043750739198 29.218321532634384 21.2611716619529 13 19.626882558024576 30.235135964106114 29.63126044459245 20.506721033276857 14 19.779089003010515 29.653185035531475 29.397332512790165 21.170393448667838 15 19.796021143799862 30.536989344423795 28.883194526812062 20.78379498496429 16 19.68696304709252 29.453462276566416 29.40214543493871 21.457429241402355 17 20.2069080879569 29.582243654765872 29.615955418331758 20.594892838945473 18 19.795022653769927 29.57074632348236 29.73348613512214 20.90074488762557 19 20.156366593228313 29.173462737841394 29.37546742765788 21.294703241272412 20 20.037908869361925 29.14171906512644 29.9495678155443 20.870804249967335 21 20.49224213401964 29.04819264400308 29.562908505349466 20.896656716627817 22 20.013976197149606 29.83324616375327 29.47367340392124 20.679104235175885 23 19.69094088234256 30.388722446044603 29.336047047843984 20.584289623768854 24 19.96752235387134 29.565303849657216 29.196340688735923 21.270833107735527 25 20.259225902845206 29.244953191221256 29.962681235648255 20.533139670285287 26 20.0021734867556 29.543611419983662 29.30251486776149 21.15170022549925 27 20.126264359796174 29.134729437132002 28.99507029097719 21.74393591209464 28 20.560252747970114 29.769835695811018 29.161292283173175 20.508619273045696 29 21.051365852303093 29.019800277837764 29.342449217877814 20.586384651981334 30 20.263352521996918 29.02061016068009 29.83181717291149 20.884220144411504 31 20.24070448088806 29.436325003224013 28.98970299875779 21.333267517130146 32 20.19718122985232 29.74454217697126 29.547760960471496 20.510515632704923 33 20.27026342850842 29.339513263682775 29.148374582872982 21.241848724935814 34 20.15964540621068 29.519055309216103 28.876892752931766 21.44440653164145 35 20.088602071056886 29.95113399315873 29.113575670587394 20.84668826519699 36 20.976637455632773 29.63023394051887 28.77432654787019 20.618802055978172 37 20.17438942178375 30.133234970057725 29.30388568701796 20.388489921140565 38 20.485786875070414 29.17373978871848 29.658193266880094 20.682280069331014 39 20.473770313993185 28.96095698305891 29.22158045641886 21.34369224652904 40 20.15476041291759 29.525869374031572 29.347376480765984 20.97199373228485 41 20.47273915766907 29.11749244297459 29.977954046647525 20.431814352708813 42 20.68338502606394 29.079904094604448 29.07458664571948 21.16212423361213 43 20.132277063195495 29.310132180026077 30.172032749043304 20.385558007735124 44 20.193205251919395 29.132749661697392 29.108948912789668 21.56509617359355 45 20.72859933577755 29.116022013956577 29.52961014454976 20.625768505716113 46 20.054022551784858 29.045519528173415 30.090395354656984 20.810062565384747 47 20.211605848490763 29.904659092123687 29.183273599940595 20.700461459444956 48 20.03753365460964 28.942619672985714 30.407858492889577 20.61198817951507 49 20.80667048488783 29.298579081624045 29.48076505449967 20.413985378988457 50 20.2247800987815 29.309904956767973 29.295728216130033 21.169586728320485 51 20.16357388876801 29.21988411066631 29.244018931488476 21.372523069077204 >>END_MODULE >>Per base GC content pass #Base %GC 1 41.70377844538889 2 39.33776484772253 3 39.72848384070456 4 40.38994832743779 5 40.37732548172614 6 41.16983935701742 7 39.6083556458353 8 40.24670016624155 9 41.264020874348184 10 41.03947312353136 11 40.87491915394986 12 41.08163471662642 13 40.13360359130144 14 40.94948245167836 15 40.57981612876415 16 41.14439228849488 17 40.801800926902374 18 40.6957675413955 19 41.45106983450073 20 40.908713119329256 21 41.38889885064746 22 40.69308043232549 23 40.27523050611141 24 41.238355461606865 25 40.79236557313049 26 41.15387371225484 27 41.87020027189081 28 41.06887202101581 29 41.637750504284426 30 41.147572666408415 31 41.5739719980182 32 40.70769686255724 33 41.512112153444235 34 41.604051937852134 35 40.93529033625388 36 41.59543951161094 37 40.56287934292431 38 41.168066944401424 39 41.81746256052223 40 41.12675414520244 41 40.90455351037788 42 41.845509259676064 43 40.517835070930616 44 41.75830142551294 45 41.35436784149366 46 40.8640851171696 47 40.912067307935715 48 40.649521834124705 49 41.22065586387629 50 41.39436682710199 51 41.53609695784522 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 95.0 1 149.0 2 203.0 3 317.0 4 431.0 5 629.0 6 827.0 7 1215.5 8 1604.0 9 2461.0 10 3318.0 11 4782.5 12 6247.0 13 8983.0 14 11719.0 15 16327.0 16 20935.0 17 27968.5 18 35002.0 19 45336.5 20 55671.0 21 69762.5 22 83854.0 23 101703.0 24 119552.0 25 142091.5 26 193169.0 27 221707.0 28 256925.5 29 292144.0 30 333005.5 31 373867.0 32 414901.0 33 455935.0 34 489696.0 35 523457.0 36 537266.0 37 551075.0 38 551087.5 39 551100.0 40 535541.0 41 519982.0 42 510659.5 43 501337.0 44 479079.0 45 456821.0 46 439148.5 47 421476.0 48 400971.5 49 380467.0 50 358697.0 51 336927.0 52 319559.0 53 302191.0 54 257606.5 55 213022.0 56 183273.5 57 153525.0 58 131684.0 59 109843.0 60 93112.0 61 76381.0 62 63844.5 63 51308.0 64 42929.5 65 34551.0 66 29002.5 67 23454.0 68 19729.0 69 16004.0 70 13797.5 71 11591.0 72 9981.0 73 8371.0 74 7318.5 75 5381.5 76 4497.0 77 3813.5 78 3130.0 79 2661.5 80 2193.0 81 1831.5 82 1470.0 83 1253.0 84 1036.0 85 811.0 86 586.0 87 509.0 88 432.0 89 337.5 90 243.0 91 193.0 92 143.0 93 105.5 94 68.0 95 49.5 96 31.0 97 23.0 98 15.0 99 8.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025426337663733027 2 0.0 3 0.0 4 0.0 5 8.437943914955651E-5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.546956384408536E-4 17 1.3830915135800972 18 0.0 19 1.546956384408536E-4 20 0.0 21 0.002981406849950997 22 0.0010688062292277159 23 0.002643889093352771 24 0.0 25 0.001476640185117239 26 0.0976551375757534 27 0.028140542956377097 28 0.041416241382573986 29 0.2951592781451487 30 0.018718172251343288 31 3.656442363147449E-4 32 0.15432999420453886 33 0.0027142052926440677 34 0.012024070078811802 35 9.563003103616405E-4 36 0.01860566633247721 37 0.03365333298081479 38 0.015132046087487134 39 0.033723649180106084 40 0.009141105907868622 41 0.0018985373808650214 42 0.029040590307305696 43 7.875414320625275E-4 44 0.02414658283663142 45 0.027760835480204093 46 0.02607324669721296 47 0.0035579996841396326 48 0.019463523963831036 49 0.013627279422653377 50 0.007017556689271449 51 0.009534876623899886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 7110737.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 10.999552685829864 #Duplication Level Relative count 1 100.0 2 6.056848166337975 3 0.8915645537533666 4 0.42229129905107377 5 0.284076941146906 6 0.2004927642325279 7 0.16716835382875622 8 0.13602915066457616 9 0.11089926740927293 10++ 0.9909914831547837 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC 23240 0.3268296943059489 TruSeq Adapter, Index 7 (100% over 51bp) ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA 11030 0.15511753563660138 No Hit TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC 8359 0.11755462197519048 No Hit >>END_MODULE >>Kmer Content fail #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CACAC 746670 2.8640375 11.838825 12 CCAGG 472520 2.7297623 6.783471 5 CCCAG 452295 2.533338 6.870746 34 CAGGG 407460 2.427856 6.6234717 6 CCCTG 417165 2.328358 6.4997187 38 TCCAG 541880 2.1362739 10.492001 25 CTCCC 389285 2.106572 6.3039412 44 GGAAG 491285 2.0676801 10.8225155 5 CAGGC 352015 2.0336013 6.238462 36 GGGGA 327920 2.0152972 6.290072 19 CTGAA 707175 1.9692125 7.6808558 19 CCAGA 483650 1.9134384 10.753026 33 CTCCA 500030 1.9112461 10.450109 24 GCACA 434390 1.7185539 10.689144 11 AGAGC 407690 1.6635914 10.564649 8 CACCA 431600 1.6555086 10.110297 31 GAAGA 566620 1.6331286 7.8772197 6 GAGCA 388255 1.5842861 10.571347 9 AAGAG 547890 1.5791446 7.826525 7 TCTGA 545965 1.5149597 7.1180396 18 GTCTG 366660 1.4856658 9.463361 17 ACCAG 374755 1.4826229 10.259815 32 AGCAC 374020 1.4797151 10.420082 10 CCAGT 370195 1.4594337 9.666598 26 CATCT 536835 1.4442545 7.285849 39 CAGTC 340195 1.3411634 9.5952 27 CAGAT 470175 1.309258 7.5189104 34 ATGCC 318140 1.2542152 9.282432 47 TCACC 322620 1.2331384 9.697449 30 ACTCC 322315 1.2319725 9.987052 23 AACTC 435225 1.175023 7.2943945 22 AGTCA 419430 1.1679525 6.9326677 28 GTCAC 288120 1.1358662 9.584073 29 ATCTC 413795 1.1132385 7.0414457 40 TCATC 406960 1.0948502 6.97533 38 GACCC 194560 1.089745 5.3296366 42 GAACT 381460 1.0622206 6.7973266 21 TGAAC 356540 0.99282795 6.7299647 20 CACGT 246410 0.9714314 9.6038 14 ATCAT 497290 0.94498444 5.0879946 37 AGATC 329515 0.9175736 7.0488634 35 GTATG 290200 0.8305521 6.2928047 45 CGGGG 94800 0.8248362 6.9011135 18 GATCA 295925 0.8240382 6.8803177 36 TATGC 289460 0.8032021 6.3008246 46 ACGTC 187745 0.7401541 9.258811 15 GGCGG 82875 0.7210791 7.050219 38 CGGAA 148395 0.6055303 8.899575 4 TCTCG 151530 0.59528226 8.973797 41 ACACG 129745 0.51330316 9.236896 13 CGTCT 114005 0.4478661 8.960011 16 CTCGT 110545 0.4342736 8.56555 42 TCGGA 95485 0.38825965 8.806305 3 ATCGG 83720 0.34042096 8.812026 2 GATCG 82740 0.3364361 9.021382 1 CGTAT 94325 0.26173577 5.8062787 44 TCGTA 85705 0.23781674 5.7963967 43 >>END_MODULE