##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename SV24_CGATGT_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13156606 Filtered Sequences 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.457883742965322 31.0 30.0 31.0 25.0 34.0 2 30.331748020728142 31.0 30.0 31.0 27.0 34.0 3 30.71551614451326 31.0 30.0 31.0 27.0 34.0 4 32.53072958177816 35.0 32.0 37.0 25.0 37.0 5 33.19110544163137 35.0 33.0 37.0 28.0 37.0 6 33.41499791055535 35.0 33.0 37.0 28.0 37.0 7 33.428342461574054 35.0 33.0 37.0 28.0 37.0 8 33.61895058649625 35.0 33.0 37.0 28.0 37.0 9 35.083285537318666 37.0 34.0 39.0 29.0 39.0 10 34.96984108211495 37.0 34.0 39.0 28.0 39.0 11 34.96539153030805 37.0 34.0 39.0 28.0 39.0 12 35.13538598024445 37.0 34.0 39.0 29.0 39.0 13 34.87420159880139 37.0 34.0 39.0 27.0 39.0 14 35.71154939199365 38.0 34.0 40.0 27.0 41.0 15 36.11844141262572 38.0 34.0 40.0 30.0 41.0 16 35.78044672007355 38.0 34.0 40.0 27.0 41.0 17 35.02919947591347 38.0 33.0 40.0 27.0 41.0 18 35.42830430583693 38.0 34.0 40.0 27.0 41.0 19 35.34268214766027 38.0 34.0 40.0 27.0 41.0 20 35.55394597968503 38.0 34.0 40.0 27.0 41.0 21 35.09534579054811 38.0 33.0 40.0 27.0 41.0 22 35.1477555837729 38.0 33.0 40.0 27.0 41.0 23 35.3342927499691 38.0 34.0 40.0 27.0 41.0 24 35.66634943692925 38.0 34.0 40.0 27.0 41.0 25 35.740907115406515 38.0 34.0 40.0 27.0 41.0 26 35.162221168590136 38.0 34.0 40.0 27.0 41.0 27 34.644676902234515 37.0 33.0 40.0 25.0 40.0 28 34.143094959292696 37.0 32.0 40.0 24.0 40.0 29 34.0356922598427 37.0 32.0 39.0 24.0 40.0 30 34.35644527167569 37.0 32.0 39.0 25.0 40.0 31 34.43013464110729 37.0 33.0 39.0 25.0 40.0 32 34.45183985900315 37.0 33.0 39.0 25.0 40.0 33 34.191879197416114 37.0 32.0 39.0 24.0 40.0 34 34.90006199167171 37.0 33.0 40.0 25.0 40.0 35 34.33251318767165 37.0 33.0 40.0 24.0 40.0 36 34.9140125500452 38.0 33.0 40.0 25.0 40.0 37 34.543844058262444 37.0 33.0 40.0 24.0 40.0 38 34.65036681952777 37.0 33.0 40.0 25.0 40.0 39 34.07711646909545 37.0 32.0 40.0 24.0 40.0 40 33.69121580444075 37.0 31.0 40.0 21.0 40.0 41 34.080934095009 37.0 32.0 40.0 24.0 40.0 42 34.068227626486646 37.0 32.0 40.0 24.0 40.0 43 33.912347074921904 37.0 32.0 40.0 23.0 40.0 44 33.828712131380996 37.0 32.0 40.0 23.0 40.0 45 33.48083479888354 37.0 31.0 40.0 21.0 40.0 46 33.087639471760426 37.0 30.0 40.0 18.0 40.0 47 33.612490637783026 37.0 31.0 40.0 23.0 40.0 48 32.97391204084093 36.0 30.0 39.0 18.0 40.0 49 33.07348224914541 36.0 30.0 39.0 19.0 40.0 50 33.50080423476997 37.0 31.0 39.0 23.0 40.0 51 32.60171430230562 36.0 30.0 39.0 19.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 6.0 10 13.0 11 28.0 12 42.0 13 90.0 14 231.0 15 561.0 16 1250.0 17 2852.0 18 5712.0 19 10682.0 20 18428.0 21 30401.0 22 48277.0 23 74017.0 24 109040.0 25 156592.0 26 219014.0 27 298651.0 28 394753.0 29 512748.0 30 647087.0 31 800459.0 32 959665.0 33 1124912.0 34 1294886.0 35 1445919.0 36 1546310.0 37 1530294.0 38 1323849.0 39 599825.0 40 9.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.45386623114906 26.50664760136759 29.352020326925583 23.68746584055777 2 20.16611274974716 28.828377166573205 29.032039114038987 21.97347096964065 3 21.7097327380633 28.19909633229117 29.337817063154432 20.7533538664911 4 21.789563356993437 26.421388616486652 30.62346018418428 21.16558784233563 5 20.873103927070524 28.68052318376152 29.141136073063244 21.305236816104706 6 21.403513945769905 28.85727519696189 27.93360992949093 21.80560092777727 7 20.966896781738388 28.93664977122519 28.853071985282526 21.24338146175389 8 20.86171007933201 28.834077724908692 28.31736391589138 21.986848279867925 9 21.216338013010347 28.366593937676633 28.620428399239135 21.79663965007389 10 21.25064777344552 28.730852014569713 28.044223563432695 21.974276648552067 11 21.155243229142833 28.399892799100314 28.429322881600317 22.015541090156535 12 21.138339173492007 28.43000694860057 28.156767786464076 22.274886091443342 13 20.836908850200427 28.737989113605742 28.44445596379492 21.980646072398915 14 21.0076991746731 28.651912860498584 28.128958800541632 22.211429164286685 15 21.16649233092486 28.86359141559761 27.964377743013664 22.005538510463868 16 21.02188890012957 28.49405282240206 28.25142132334269 22.23263695412568 17 21.083082461040622 28.4324885745414 28.510258599976172 21.974170364441807 18 21.146426365583952 28.418149787262763 28.395864404543236 22.039559442610045 19 20.89938799496009 28.20228279498846 28.695480462986193 22.202848747065254 20 21.14594313243752 28.134783109683926 28.656452683382426 22.062821074496124 21 21.161409819151615 28.050764240226467 28.71623406301589 22.07159187760603 22 20.82464684226224 28.41137772486243 28.626253416196658 22.13772201667867 23 20.959268121383857 28.532343489192996 28.461556002152584 22.046832387270566 24 21.364651339410788 28.364313714342437 28.063787879640085 22.20724706660669 25 21.41342024032183 28.288873867940424 28.38933416719613 21.908371724541613 26 21.355538288742885 28.420827914841663 28.175903088496508 22.047730707918948 27 21.441617035757822 28.229843505065627 28.2056888752914 22.122850583885146 28 21.40377562906557 28.420319374764137 28.394876699977107 21.781028296193185 29 21.73062111467924 28.116458697447165 28.271635222273584 21.88128496560001 30 21.638749915424903 28.118791332515837 28.23843563815998 22.004023113899283 31 21.58512428768278 28.1079445473971 28.102677202269017 22.2042539626511 32 21.566815463662167 28.43205793993932 28.17401407971461 21.827112516683904 33 21.607911098949543 28.13826940909989 28.09195664401575 22.161862847934813 34 21.5537876619677 28.32747055020462 27.984263035808016 22.134478752019668 35 21.59774966719756 28.302025758612466 28.421738866718947 21.678485707471026 36 21.65217751582302 28.698029016819476 27.922574681116412 21.72721878624109 37 21.423374582931277 28.28271661437254 28.591820583143107 21.70208821955308 38 21.83598106546984 28.25748060125929 28.118221235460684 21.788317097810186 39 21.536466415867185 28.028218211059336 28.648546229286726 21.786769143786753 40 21.35507939652288 28.172634093991483 28.59379316205818 21.87849334742746 41 21.60593436642902 28.13513296579377 28.4725830544052 21.78634961337201 42 21.607979301186187 28.154151756378532 28.189269671297378 22.048599271137903 43 21.471667015797856 28.152411422275343 28.616396133065503 21.759525428861302 44 21.433909533725476 28.22802604039561 28.27495683628808 22.063107589590835 45 21.648645870523968 28.150007694090267 28.48695866905011 21.714387766335648 46 21.293795764307603 28.138259442032304 28.864246501991502 21.703698291668587 47 21.555679435771054 28.526487909238607 28.094725494938537 21.8231071600518 48 21.236522559114636 28.00960132485755 29.016130280496803 21.737745835531015 49 21.70470919539225 28.125280553592813 28.426683958206873 21.74332629280806 50 21.642024132461504 28.15762455056352 28.15423436987981 22.046116947095165 51 21.545290007928344 27.99345968516183 28.37587502555998 22.08537528134984 >>END_MODULE >>Per base GC content pass #Base %GC 1 44.141332071706834 2 42.139583719387815 3 42.4630866045544 4 42.955151199329066 5 42.17834074317523 6 43.20911487354717 7 42.21027824349228 8 42.848558359199934 9 43.012977663084236 10 43.22492442199759 11 43.17078431929937 12 43.41322526493535 13 42.817554922599335 14 43.219128338959784 15 43.17203084138873 16 43.254525854255256 17 43.05725282548243 18 43.185985808194 19 43.10223674202535 20 43.208764206933644 21 43.23300169675765 22 42.96236885894091 23 43.00610050865442 24 43.57189840601748 25 43.32179196486345 26 43.40326899666183 27 43.56446761964297 28 43.184803925258755 29 43.61190608027925 30 43.64277302932418 31 43.78937825033388 32 43.39392798034607 33 43.769773946884364 34 43.688266413987364 35 43.27623537466859 36 43.37939630206411 37 43.12546280248436 38 43.62429816328003 39 43.32323555965394 40 43.23357274395034 41 43.392283979801036 42 43.65657857232409 43 43.23119244465916 44 43.49701712331631 45 43.36303363685962 46 42.99749405597619 47 43.37878659582286 48 42.974268394645655 49 43.44803548820031 50 43.68814107955667 51 43.630665289278184 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 76.0 1 110.0 2 144.0 3 209.0 4 274.0 5 389.5 6 505.0 7 776.0 8 1047.0 9 1614.5 10 2182.0 11 3378.0 12 4574.0 13 6808.0 14 9042.0 15 13030.5 16 17019.0 17 23911.5 18 30804.0 19 41848.0 20 52892.0 21 68977.5 22 85063.0 23 107712.5 24 130362.0 25 162586.5 26 241025.0 27 287239.0 28 352843.0 29 418447.0 30 502736.0 31 587025.0 32 677745.5 33 768466.0 34 848030.0 35 927594.0 36 963258.0 37 998922.0 38 1001063.5 39 1003205.0 40 972564.5 41 941924.0 42 924754.0 43 907584.0 44 888694.5 45 869805.0 46 851976.5 47 834148.0 48 807995.0 49 781842.0 50 745260.0 51 708678.0 52 662869.5 53 617061.0 54 552415.5 55 487770.0 56 433398.5 57 379027.0 58 332719.0 59 286411.0 60 249016.5 61 211622.0 62 183550.5 63 155479.0 64 135445.0 65 115411.0 66 100995.5 67 86580.0 68 75746.5 69 64913.0 70 57346.5 71 49780.0 72 44104.5 73 38429.0 74 33891.5 75 25893.0 76 22432.0 77 19451.0 78 16470.0 79 13968.5 80 11467.0 81 9628.0 82 7789.0 83 6475.5 84 5162.0 85 4226.0 86 3290.0 87 2667.5 88 2045.0 89 1631.0 90 1217.0 91 947.0 92 677.0 93 507.5 94 338.0 95 242.5 96 147.0 97 98.0 98 49.0 99 30.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024436393398114986 2 0.0 3 0.0 4 0.0 5 3.8003722236570736E-5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 7.600744447314148E-6 15 0.0 16 2.2042158897211028E-4 17 1.367252314160658 18 0.0 19 1.1401116670971223E-4 20 1.5201488894628297E-5 21 0.002561450878744868 22 0.001231320600464892 23 0.0024778426898244125 24 0.0 25 0.0012541228338068342 26 0.09556415993608078 27 0.02924766463326484 28 0.04159887436015033 29 0.288813087509043 30 0.01988354747417381 31 5.548543446539328E-4 32 0.1496738596565102 33 0.0030859022456095437 34 0.01209278441567681 35 0.0011553131559917504 36 0.019085469307205824 37 0.03342047333484031 38 0.015315500061338007 39 0.034446573835227715 40 0.0094553260924588 41 0.002204215889721103 42 0.02869281028861091 43 0.0010489027337293524 44 0.024291979253616015 45 0.027773120210485898 46 0.02644298993220592 47 0.00401319306818187 48 0.019670726629649013 49 0.01376494819408592 50 0.007334718391658153 51 0.009698549914772852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1.3156606E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 9.680807098577851 #Duplication Level Relative count 1 100.0 2 2.5117891518270494 3 0.7977864387071768 4 0.5609267672961392 5 0.42042591675459184 6 0.33860166663077884 7 0.2917680497835964 8 0.2352447191059624 9 0.20133072069938202 10++ 2.3056135742124417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC 15224 0.11571373346591059 TruSeq Adapter, Index 2 (100% over 51bp) >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position GAAAA 2558280 3.0408795 3.3972387 4 CACAC 1500840 2.879384 5.993513 12 >>END_MODULE